一、中文名称：Anti-Hypusine antibody Hpu24 单克隆抗体，海普辛抗体
二、产品描述
hypusine是在真核细胞中的特定蛋白质——即真核翻译起始因子5A (eIF5A) 上发现的。这种氨基酸是通过赖氨酸残基经过一系列酶促反应生成的，包括脱氧hypusine合酶(DHPS) 和脱氧hypusine羟化酶(DOHH) 的作用。首先，DHPS催化一个丁胺基团转移到赖氨酸上形成脱氧hypusine，随后DOHH将脱氧hypusine上的胺基氧化成羟基，从而生成hypusine。Hypusine的存在对于eIF5A的功能至关重要，因为它是唯一含有这种独特氨基酸残基的蛋白质。eIF5A在多种生物过程中发挥作用，例如细胞增殖、蛋白质合成、能量代谢以及炎症反应等。此外，研究表明，hypusine可能影响T细胞分化和巨噬细胞激活。最近的研究还揭示了游离hypusine具有潜在的生物活性，并能抑制肿瘤细胞增殖。
三、产品详情
名称：Anti-Hypusine antibody (Hpu24) (RGK08101)
别名：Hpu24，单克隆抗体，Hypusine, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A
货号：RGK08101
表达系统：Mammalian Cells
种属反应性：General
宿主：Rabbit
同种型：IgG
克隆类型：Monoclonal
经测试应用：ELISA: 1:2000-1:8000, IHC: 1:50-1:100, WB: 1:500-1:4000
靶标：Hypusine, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A
浓度：1 mg/ml
内毒素水平：Please contact with the lab for this information.
纯度：>95% as determined by SDS-PAGE.
纯化方式：Protein A/G purified from cell culture supernatant.
Accession号：PubChem: 65396
克隆号：Hpu24
应用：ELISA, IHC, WB
状态：Liquid
保存溶液：0.01M PBS, pH 7.4.
稳定性和存储：Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. (RGK08101)
四、参考文献：
Helicobacter pylori in gastric cancer and peptic ulcer disease in a Colombian population. Strain heterogeneity and antibody profiles. PMID: 11683922
A dual-function [Ru(bpy)3]2+ encapsulated metal organic framework for ratiometric Al3+ detection and anticounterfeiting application. PMID: 36866710
Hypusine, a polyamine-derived amino acid critical for eukaryotic translation. PMID: 30257869
Post-translational formation of hypusine in eIF5A: implications in human neurodevelopment. PMID: 34273022
Role of Polyamines and Hypusine in beta Cells and Diabetes Pathogenesis. PMID: 35448531
The hypusine-containing translation factor eIF5A. PMID: 25029904
Modulating the polyamine/hypusine axis controls generation of CD8+ tissue-resident memory T cells. PMID: 37581943
Hypusine: its post-translational formation in eukaryotic initiation factor 5A and its potential role in cellular regulation. PMID: 8347280
The Polyamine-Hypusine Circuit Controls an Oncogenic Translational Program Essential for Malignant Conversion in MYC-Driven Lymphoma. PMID: 37070973
Targeting the polyamine-hypusine circuit for the prevention and treatment of cancer. PMID: 27357307
Hypusine is essential for eukaryotic cell proliferation. PMID: 9285094
Functional significance of eIF5A and its hypusine modification in eukaryotes. PMID: 19997760
Hypusine: a new target for therapeutic intervention in diabetic inflammation. PMID: 20670594
Is hypusine essential for eukaryotic cell proliferation? PMID: 8108861
Posttranslational synthesis of hypusine: evolutionary progression and specificity of the hypusine modification. PMID: 17476569
Spermidine-induced hypusination preserves mitochondrial and cognitive function during aging. PMID: 34105442
Hypusine Signaling Promotes Pulmonary Vascular Remodeling in Pulmonary Arterial Hypertension. PMID: 38261723
Polyamine metabolism is a central determinant of helper T cell lineage fidelity. PMID: 34216540
Hypusine modification of the ribosome-binding protein eIF5A, a target for new anti-inflammatory drugs: understanding the action of the inhibitor GC7 on a murine macrophage cell line. PMID: 23701550
Translation Factor eIF5A, Modification with Hypusine and Role in Regulation of Gene Expression. eIF5A as a Target for Pharmacological Interventions. PMID: 30208826
Hypusine biosynthesis in protein and its biological consequences. PMID: 3151230
Biochemistry and function of hypusine formation on eukaryotic initiation factor 5A. PMID: 9285092
Biosynthesis of hypusine in eIF-4D precursors. PMID: 3151229
YAP/TAZ drives cell proliferation and tumour growth via a polyamine-eIF5A hypusination-LSD1 axis. PMID: 35177822
Spermidine-mediated hypusination of translation factor EIF5A improves mitochondrial fatty acid oxidation and prevents non-alcoholic steatohepatitis progression. PMID: 36057633
Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation. PMID: 31130465
Arginine metabolism regulates human erythroid differentiation through hypusination of eIF5A. PMID: 36735910
The post-translational synthesis of a polyamine-derived amino acid, hypusine, in the eukaryotic translation initiation factor 5A (eIF5A). PMID: 16452303
The first evidence of biological activity for free Hypusine, an enigmatic amino acid discovered in the '70s. PMID: 37258638
Hypusine biosynthesis in beta cells links polyamine metabolism to facultative cellular proliferation to maintain glucose homeostasis. PMID: 31796630
Polyamines. PMID: 6206782
Deoxyhypusine hydroxylase as a novel pharmacological target for ischemic stroke via inducing a unique post-translational hypusination modification. PMID: 35007708
Pharmacological targeting of polyamine and hypusine biosynthesis reduces tumour activity of endometrial cancer. PMID: 35100927
Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. PMID: 31474573
At the hypusine of the crime. PMID: 22763665
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