一、中文名称：Anti-DNA-RNA Hybrid Antibody(S9.6) (RGK60001)，抗-DNA-RNA杂交抗体
二、产品描述
DNA-RNA杂交体在真核细胞内是一种自然现象，并且在高转录活性位点其水平较高。它们是非经典的核酸结构，具有转录调控功能。据报道，它们的存在会使某个基因座易于发生染色体断裂。形成DNA:RNA的基因座会创建一个双链A/B中间构象，在互补的、被置换的DNA链上提供第二个单链核酸结合蛋白的目标。这些杂交体已被证明对DNA甲基转移酶的活性具有抗性。DNA:RNA杂交体的形成与多种神经系统疾病有关。DNA:RNA解旋酶senataxin（SETX）的突变与显性青少年肌萎缩侧索硬化症4型和隐性眼动失协调2型相关。
三、产品详情
名称：Anti-DNA-RNA Hybrid Antibody(S9.6) (RGK60001)
货号：RGK60001
表达系统：Mammalian Cells
种属反应性：General
宿主：mouse
同种型：IgG2a, kappa
克隆类型：Monoclonal
靶标：DNA-RNA Hybrid
浓度：0.58 mg/ml
内毒素水平：Please contact with the lab for this information.
纯度：>95% as determined by SDS-PAGE.
纯化方式：Protein A/G purified from cell culture supernatant.
克隆号：S9.6
应用：CHIP, ChIP-seq, DB, EMSA, FISH, ICC, IF, IP, SPR
状态：Liquid
保存溶液：0.01M PBS, pH 7.4.
稳定性和存储：Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
四、参考文献：
Recognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids. PMID: 33830170
Immunoprecipitation of DNA:RNA Hybrids Using the S9.6 Antibody. PMID: 32681514
High-resolution, strand-specific R-loop mapping via S9.6-based DNA-RNA immunoprecipitation and high-throughput sequencing. PMID: 31053798
RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage. PMID: 29742442
The monoclonal S9.6 antibody exhibits highly variable binding affinities towards different R-loop sequences. PMID: 28594954
Structural basis of R-loop recognition by the S9.6 monoclonal antibody. PMID: 35347133
Best practices for the visualization, mapping, and manipulation of R-loops. PMID: 33411340
Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA-DNA hybrid imaging. PMID: 34232287
The Affinity of the S9.6 Antibody for Double-Stranded RNAs Impacts the Accurate Mapping of R-Loops in Fission Yeast. PMID: 29289567
The sub-nanomolar binding of DNA-RNA hybrids by the single-chain Fv fragment of antibody S9.6. PMID: 23784994
Modifications and interactions at the R-loop. PMID: 32961406
BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2. PMID: 24896180
R-Loop Analysis by Dot-Blot. PMID: 33554969
Amplification-Free and Mix-and-Read Analysis of Multiplexed MicroRNAs on a Single Plasmonic Microbead. PMID: 34324345
New insight into the biology of R-loops. PMID: 32516653
Detection of Telomeric DNA:RNA Hybrids Using TeloDRIP-qPCR. PMID: 33371452
Ultra-deep Coverage Single-molecule R-loop Footprinting Reveals Principles of R-loop Formation. PMID: 32105733
Complexities due to single-stranded RNA during antibody detection of genomic rna:dna hybrids. PMID: 25890199
Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening. PMID: 34495394
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