一、中文名称：Benzonase Nuclease全能核酸酶，YXX06401
二、产品描述
全能核酸酶，又称广谱核酸酶，一种来源于Serratia Marcescen的酶。它可以降解所有形式的DNA和RNA，包括单链、双链、线状、环状、天然以及变性的核酸，将它们消化成3-8个碱基长度的5-单磷酸寡核苷酸，且不具有碱基识别特异性。另外，全能核酸酶在非常广泛的条件下都能保持很高的稳定性和消化活力，非常适用于作为各类科学研究以及疫苗、蛋白、多糖类制药工业的首选酶制剂，去除样本或制品中的核酸残留，提升样本纯度和制品生物功效。
三、产品详情
货号: YXX06401
生物学活性: 37 ℃, pH 8.0 reaction conditions, 2.625 mL reaction system, change the absorption value of △ A260 to 1.0 in 30 minutes（equivalent to the complete digestion of 37 μg salmon sperm DNA into an oligonucleotide). The amount of enzyme used is defined as an activity Unit（U)
表达系统: E.coli
种属: Serratia marcescens
蛋白长度: Benzonase Nuclease is cloned from Serratia marcescens and expressed in E.coli.
预测分子量: 30.16 kDa
性质: Recombinant
靶点: Benzonase Nuclease/Endonuclease
浓度: 1000U/μL
内毒素水平: Please contact with the lab for this information.
纯度: >90% as determined by SDS-PAGE.
形态: Liquid
储存缓冲液: 25 mM Tris pH 8.0, 100mM NaCI, 50% Glycerol.
运输方式: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性与储存: Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
别名: Benzonase Nuclease, Endonuclease
四、参考文献：
1. Isoforms of Serratia marcescens nuclease. The role of Mg2+ in the hydrolysis mechanism. PMID: 9457762
2. Evaluation of the Serratia marcescens nuclease (NucA) as a transgenic cell ablation system in porcine. PMID: 19937494
3. Optimisation of SARS-CoV-2 peptide stimulation and measurement of cytokine output by intracellular flow cytometry and bio-plex analysis. PMID: 37683822
4. Nucleic acids in prion preparations: unspecific background or essential component? PMID: 7913761
5. Cleavage experiments with deoxythymidine 3',5'-bis-(p-nitrophenyl phosphate) suggest that the homing endonuclease I-PpoI follows the same mechanism of phosphodiester bond hydrolysis as the non-specific Serratia nuclease. PMID: 9989607
6. Action of hexaamminecobalt on the activity of Serratia marcescens nuclease. PMID: 12680708
7. Antiretroviral effect of a gag-RNase HI fusion gene. PMID: 9231076
8. [Expression, purification and characterization of non-specific Serratia nuclease in Escherichia coli]. PMID: 22097815
9. Microtiter-plate assay and related assays for nonspecific endonucleases. PMID: 11265297
10. The DNA/RNA non-specific Serratia nuclease prefers double-stranded A-form nucleic acids as substrates. PMID: 10329148
11. Efficient removal of DNA from proteomic samples prior to two-dimensional map analysis. PMID: 19081104
12. 2.1 A structure of Serratia endonuclease suggests a mechanism for binding to double-stranded DNA. PMID: 7664065
13. The extracellular nuclease gene of Serratia marcescens and its secretion from Escherichia coli. PMID: 3319779
14. A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli. PMID: 8167472
15. Quantitative binding studies of a monoclonal antibody to immobilized protein-A. PMID: 1368207
16. Analysis of the reaction mechanism of the non-specific endonuclease of Serratia marcescens using an artificial minimal substrate. PMID: 8955376
17. Performance of pre-RT-qPCR treatments to discriminate infectious human rotaviruses and noroviruses from heat-inactivated viruses: applications of PMA/PMAxx, benzonase and RNase. PMID: 29464825
18. Enzymatic biofilm destabilisation to support mechanical cleansing of inserted dental implant surfaces: an in-vitro pilot study. PMID: 33740161
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