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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHE00201 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHE00201 |
| 产品名称 | Recombinant Human CDA Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P32320 |
| 蛋白长度 | Ala2-Gln146 |
| 预测分子量 | 18.36 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 别名 | Cytidine deaminase, CDD, Cytidine aminohydrolase, CDA |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
CDA 属于嘧啶代谢关键水解酶,可催化胞苷、脱氧胞苷脱氨基转化为尿苷与脱氧尿苷,参与核苷回收循环,维持细胞内核苷酸储备平衡,广泛存在于各类体细胞胞质中。
Q1: 这款CDA蛋白分子量仅18.36 kDa,做Western Blot时有什么注意事项?
A: 由于分子量较小,建议使用15%高浓度分离胶并适当延长电泳时间,转膜时选用0.22 μm小孔径PVDF膜,以确保蛋白有效分离和完全转移。
Q2: 该蛋白可直接用于细胞培养或体内实验吗?内毒素如何控制?
A: 目前内毒素水平需联系实验室获取具体批次数据。若用于细胞或动物实验,建议先确认内毒素检测结果,必要时自行进行LAL验证或内毒素去除处理。
Q3: 复溶后的蛋白溶液应如何保存?能否反复冻融?
A: 复溶后建议立即分装成小体积,避免反复冻融。短期频繁使用可置于2-8°C,长期储存应置于-20°C至-80°C,每次取用单份以维持蛋白稳定性。
Q4: 用该蛋白制备抗体时,N-His标签会干扰抗体特异性吗?
A: N-His标签可能诱导针对标签的非目标抗体。若需制备特异性抗CDA抗体,建议以去除标签的蛋白为免疫原,或在筛选阶段用His标签蛋白进行阴性吸附对照,排除标签特异性抗体。
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