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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHD84101 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHD84101 |
| 产品名称 | Recombinant Human CASP1/Caspase-1 Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P29466 |
| 蛋白长度 | Asn120-Asp297 |
| 预测分子量 | 22.15 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 别名 | IL1BC, Interleukin-1 beta-converting enzyme, p45, Caspase-1, IL-1BC, IL-1 beta-converting enzyme, CASP1, IL1BCE, ICE, CASP-1, Interleukin-1 beta convertase |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
CASP1(半胱天冬酶1)属于炎性蛋白酶,负责活化炎性因子,触发细胞焦亡进程,免疫细胞中大量表达。蛋白活性失衡会打乱细胞防御机制,是细胞焦亡机制、免疫防御调控方向常用研究靶点。
Q1:CASP1 蛋白表达体系与质控标准是什么?
A:大肠杆菌表达、N 端 His 标签,分子量 22.15 kDa,SDS-PAGE 纯度>90%。
Q2:冻干蛋白复溶有什么要求?
A:添加无菌水配制储备液,详细配比与操作步骤参照配套说明书。
Q3:如何储存可维持蛋白稳定?
A:短期 2~8℃存放,长期分装置于 - 20~-80℃,保质期 12 个月,不可反复冻融。
Q4:蛋白应用场景与使用限制?
A:可用于 WB、ELISA、免疫抗体制备,生物活性尚在验证,仅用于科研使用。
前沿相关研究:
1.miR-126-5p protects from URSA via inhibiting Caspase-1-dependent pyroptosis of trophoblast cells., PMID:40372489
2.Identification and validation of pyroptosis patterns in AML via comprehensive bioinformatics analysis., PMID:40208371
3.Diacerein counteracts amiodarone‑induced hepatotoxicity in rats via targeting TLR4/NF-kB/NLRP3 pathways., PMID:40331897
4.HDAC1 Promotes Hippocampal Neuronal Pyroptosis in Epileptic Mice Through the miR-15a-5p/Caspase-1 Axis., PMID:40131574
5.Aspartame increases the risk of liver cancer through CASP1 protein: A comprehensive network analysis insights., PMID:40139029
6.A tightly regulated auxin signaling landscape is required for spatial accommodation of lateral roots in Arabidopsis., PMID:40133999
7.[The HMGB1, caspase-1 and gasdermin D-mediated pyroptosis in high-altitude retinopathy: an experimental study]., PMID:40050105