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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHD41001 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHD41001 |
| 产品名称 | Recombinant Human CD11a/ITGAL Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P20701 |
| 蛋白长度 | Asn809-His1066 |
| 预测分子量 | 31.16 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | CD11A, Leukocyte function-associated molecule 1 alpha chain, Leukocyte adhesion glycoprotein LFA-1 alpha chain, LFA-1A, CD11 antigen-like family member A, CD11a, ITGAL, Integrin alpha-L |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
CD11a/ITGAL为整合素亚基蛋白,介导免疫细胞相互黏附,调控免疫细胞游走定植,主要表达于免疫细胞表面。表达失衡会打乱免疫细胞分布规律,是细胞黏附作用、免疫细胞转运方向常用研究靶点。
Q1:这款重组人 CD11a/ITGAL 蛋白的基本质控参数如何?
A:本品为大肠杆菌表达的重组人源 CD11a 蛋白,带 N-His 标签,经 SDS-PAGE 检测纯度>90%,分子量理论值 31.16 kDa,蛋白完整性与纯度可满足常规科研试验标准。
Q2:蛋白复溶和使用有什么注意要点?
A:干粉状态建议使用无菌纯水整体复溶配制母液。复溶操作、推荐工作浓度及使用细节可严格参照随货官方说明书操作,避免蛋白降解。
Q3:样品保存与稳定性如何把控?
A:短期频繁使用可 2–8℃ 暂存;长期稳定保存建议 -20℃~-80℃,自签收之日起有效期 12 个月。务必使用手动除霜冰箱,严禁反复冻融,保证蛋白结构与活性稳定。
Q4:该 CD11a 蛋白主要可用于哪些科研场景?
A:适用于Western Blot、SDS-PAGE、ELISA 检测、免疫抗原制备等实验,目前产品生物活性仍在持续验证中,仅用于科研用途,不可用于临床与人体试验。
前沿相关研究:
1.Xenorecognition and costimulation of porcine endothelium-derived extracellular vesicles in initiating human porcine-specific T cell immune responses., PMID:37054891
2.Tff3-/- Knock-Out Mice with Altered Lipid Metabolism Exhibit a Lower Level of Inflammation following the Dietary Intake of Sodium Chloride for One Week., PMID:37108475
3.Upregulation of CD226 on subsets of T cells and NK cells is associated with upregulated adhesion molecules and cytotoxic factors in patients with tuberculosis., PMID:37244120
4.CD28/PD1 co-expression: dual impact on CD8+ T cells in peripheral blood and tumor tissue, and its significance in NSCLC patients' survival and ICB response., PMID:37898752
5.Cutting Edge: Influenza-Induced CD11alo Airway CD103+ Tissue Resident Memory T Cells Exhibit Compromised IFN-γ Production after In Vivo TCR Stimulation., PMID:36912465
6.Plasma Leptin Levels, Obstructive Sleep Apnea Syndrome, and Diabetes Are Associated with Obesity-Related Alterations of Peripheral Blood Monocyte Subsets., PMID:36921085
7.Differential tumor protein expression at follicular lymphoma diagnosis reveals dysregulation of key molecular pathways associated with histological transformation., PMID:39622932