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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YMD03401 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YMD03401 |
| 产品名称 | Recombinant Mouse MIF/GIF Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a 0.2 μm filtered solution of PBS, 0.02% Tween 80, 4.0% Mannitol, 4.0% Sucrose, pH7.4. |
| 纯度 | >80% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P34884 |
| 蛋白长度 | Met1-Ala115 |
| 预测分子量 | 14.67 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | MIF, L-dopachrome isomerase, L-dopachrome tautomerase, GIF, Macrophage migration inhibitory factor, MMIF, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GLIF |
| 物种 | Mus musculus (Mouse) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
背景介绍:
MIF为多功能分泌蛋白,介导多条胞内信号通路,调控细胞迁移、增殖与免疫响应。该蛋白在各类组织广泛表达,表达失衡会破坏细胞稳态,是免疫调控相关研究的重要靶点。
Q1:这款重组小鼠 MIF/GIF 蛋白的纯度是多少?
A:本产品经 SDS-PAGE 检测,纯度大于 80%,可满足各类常规科研实验需求。
Q2:该 MIF/GIF 蛋白如何复溶配制储备液?
A:可直接使用无菌水复溶配制储备液,参照产品配套说明书开展标准操作。
Q3:这款重组 MIF/GIF 蛋白的储存条件与保质期是什么?
A:日常频繁使用可保存于 2~8℃;长期保存需置于 - 20~-80℃环境,自收货之日起保质期 12 个月。储存需使用手动除霜冰箱,避免反复冻融,防止蛋白活性流失。
Q4:该重组小鼠 MIF/GIF 蛋白可应用于哪些实验?
A:产品适用于 ELISA、免疫原制备、SDS-PAGE、WB 等实验,目前生物活性检测正在推进中,仅可用于科研用途。
前沿相关研究:
1.Integrative analysis of semaphorins family genes in colorectal cancer: implications for prognosis and immunotherapy., PMID:40103807
2.Bioinformatic analysis of serpina1 expression in papillary thyroid carcinoma and its potential association with Hashimoto's thyroiditis., PMID:40106166
3.Estrogen via GPER downregulated HIF-1a and MIF expression, attenuated cardiac arrhythmias, and myocardial inflammation during hypobaric hypoxia., PMID:40108505
4.Single-Cell Transcriptomics Identifies a Prominent Role for the MIF-CD74 Axis in Myasthenia Gravis Thymus., PMID:40117520
5.Single-cell and spatial analyses reveal the effect of VSIG4+S100A10+TAMs on the immunosuppression of glioblastoma and anti-PD-1 immunotherapy., PMID:40127797
6.MIF as an oncogenic driver of low-heterogeneity melanomas., PMID:40131169