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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHD00601 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHD00601 |
| 产品名称 | Recombinant Human CD142/F3/TF Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P13726 |
| 蛋白长度 | Met1-Glu251 |
| 预测分子量 | 30.59 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 别名 | Tissue factor, CD142, F3, Thromboplastin, Coagulation factor III, TF |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
CD142/F3/TF(组织因子)属于跨膜糖蛋白,启动凝血级联反应,调控凝血进程,多种体细胞均可表达。蛋白表达异常会打破凝血平衡,是凝血机制、生理稳态研究常用靶点。
Q1: 这款重组人CD142/TF蛋白的基本参数是什么?
A1: 该蛋白在大肠杆菌(E. coli)中表达,N端带有His标签(N-His),覆盖Met1-Glu251区段,预测分子量约30.59 kDa,纯度经SDS-PAGE验证大于90%。
Q2: 该蛋白可用于哪些实验应用?生物活性是否已验证?
A2: 目前验证可用于ELISA、免疫原制备、SDS-PAGE和Western Blot。生物活性测试正在进行中,如需latest数据请联系实验室。
Q3: 这款组织因子(TF)蛋白的内毒素水平是多少?
A3: 内毒素水平未在说明书中直接标注,如需具体检测数据,请联系实验室获取详细质检报告。
Q4: 收到冻干粉后如何正确复溶和保存?
A4: 建议使用无菌水复溶。复溶后若频繁使用可置于2-8°C短期保存,长期储存建议分装后置于-20至-80°C,避免反复冻融,保质期为收货后12个月。