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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHC97801 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHC97801 |
| 产品名称 | Recombinant Human CCL2/MCP-1 Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P13500 |
| 蛋白长度 | Gln24-Thr99 |
| 预测分子量 | 10.98 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | HC11, MCP-1, MCP1, Monocyte chemotactic protein 1, Monocyte chemotactic and activating factor, Small-inducible cytokine A2, SCYA2, MCAF, Monocyte secretory protein JE, C-C motif chemokine 2, Monocyte chemoattractant protein 1, CCL2 |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
CCL2/MCP-1属于趋化因子蛋白,招募免疫细胞定向迁移聚集,参与组织微环境重塑,多种体细胞均可分泌。表达失衡会造成免疫细胞分布失调,是细胞趋化运动、组织微环境调控方向常用研究靶点。
Q1:CCL2/MCP-1 蛋白生产与质控详情?
A:大肠杆菌原核表达,N 端 His 标签,理论分子量 10.98 kDa,电泳检测纯度>90%。
Q2:冻干粉末复溶方式?
A:无菌水溶解制备储备液,详细配比参考产品附带资料。
Q3:储存方式与保质期?
A:频繁使用 2~8℃暂存,长期分装置于 - 20~-80℃,有效期 12 个月,禁止反复冻融。
Q4:可用实验类型与使用限制?
A:支持 WB、ELISA、免疫原制备,生物活性正在检测,仅允许科研用途。
前沿相关研究:
1.Impact of Smoking on Macrophage-Related Chemokines During Initial Peri-Implantitis: A Prospective Cohort Study., PMID:40399133
2.Urinary MCP-1 and VCAM-1 as non-invasive biomarkers for the diagnosis and activity assessment of lupus nephritis., PMID:40388387
3.Alyssin Modulates Inflammatory Mediators Expression in Interleukin-1β-Stimulated Human Periodontal Ligament Cells., PMID:40384003
4.Prognostic Value of Urinary Biomarkers in Proteinuria Progression in IgA Nephropathy Patients Treated with Budesonide., PMID:40428765
5.Environmentally Relevant Lead Exposure Impacts Gene Expression in SH-SY5Y Cells Throughout Neuronal Differentiation., PMID:40397994
6.Differential effects of fentanyl compared to morphine on neuroinflammatory signaling in the brain in EcoHIV-infected mice., PMID:40447886