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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHC95001 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHC95001 |
| 产品名称 | Recombinant Human CD143/ACE Protein, N-GST |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P12821 |
| 蛋白长度 | Arg814-Gln1071 |
| 预测分子量 | 56.39 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 别名 | Dipeptidyl carboxypeptidase I, DCP, CD143, Angiotensin-converting enzyme, DCP1, ACE, Kininase II |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
CD143/ACE,膜结合蛋白酶,催化脉管紧张素转化,参与体液平衡、脉管状态调控过程,全身多处组织均有表达。蛋白功能失常会扰乱体液循环稳态,是循环生理、脉管生理方向的经典研究靶点。
Q1: 这款重组人CD143/ACE蛋白的纯度和分子量是多少?
A: 该蛋白纯度大于90%,经SDS-PAGE验证;预测分子量为56.39 kDa,适用于ELISA、免疫原制备、Western Blot等实验。
Q2: 该蛋白的表达系统和标签是什么?
A: 该蛋白在大肠杆菌(E. coli)中表达,N端带有GST标签(N-GST),便于纯化和检测,蛋白覆盖Arg814-Gln1071区段。
Q3: 这款蛋白的生物活性是否经过验证?
A: 目前生物活性测试正在进行中,该蛋白已确认可用于ELISA、免疫原、SDS-PAGE和WB等应用。
Q4: 如何正确复溶和储存该蛋白?
A: 蛋白为冻干粉形式,建议使用无菌水复溶;短期频繁使用可置于2-8°C保存,长期储存建议置于-20至-80°C,避免反复冻融。
前沿相关研究:
1.Genetic Polymorphisms and Genetic Risk Scores Contribute to the Risk of Coronary Artery Disease (CAD) in a North Indian Population., PMID:39126122
2.Sanger Sequencing Reveals Novel Variants in GLO-1, ACE, and CBR1 Genes in Patients of Early and Severe Diabetic Nephropathy., PMID:39336582
3.Association between Complex ACTN3 and ACE Gene Polymorphisms and Elite Endurance Sports in Koreans: A Case-Control Study., PMID:39336701
4.Elevated serum angiotensin converting enzyme correlates with specific HLA-DRB1 alleles and extrapulmonary manifestations in sarcoidosis., PMID:39393304
5.Computational Study of Molecular Mechanism for the Involvement of Human Serum Albumin in the Renin-Angiotensin-Aldosterone System., PMID:39408590
6.ACE and ACE2 activities and polymorphisms assessment: A populational study from Ipaussu (SP, Brazil) during the COVID-19 pandemic., PMID:39437850
7.[Polymorphism of RAAS genes in patients with COVID-19: comparison with frequency in population and relationship with severity of course]., PMID:39467241