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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHC93001 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHC93001 |
| 产品名称 | Recombinant Human RLBP1 Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P12271 |
| 蛋白长度 | Met1-Phe317 |
| 预测分子量 | 38.78 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | Retinaldehyde-binding protein 1, CRALBP, Cellular retinaldehyde-binding protein, RLBP1 |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
Q1:这款重组人RLBP1蛋白的纯度是多少?
A:本产品经SDS-PAGE检测,纯度大于90%,可满足各类常规科研实验需求。
Q2:该RLBP1蛋白如何复溶配制储备液?
A:可直接使用无菌水复溶配制储备液,可参照产品配套说明书进行标准操作。
Q3:这款重组RLBP1蛋白的储存条件与保质期是什么?
A:日常频繁使用可保存于2~8℃;长期保存需置于-20~-80℃环境,自收货之日起保质期12个月。储存需使用手动除霜冰箱,避免反复冻融,防止蛋白活性流失。
Q4:该重组人RLBP1蛋白可应用于哪些实验?
A:产品适用于ELISA、免疫原制备、SDS-PAGE、WB等实验,目前生物活性检测正在推进中,仅可用于科研用途。
背景介绍:
RLBP1(视黄醛结合蛋白 1)主要表达于视网膜色素上皮细胞,能够结合视黄醛,参与视觉循环中视黄醇转运。该蛋白维系感光细胞正常生理功能,序列功能异常易造成视网膜功能异常,是视觉生理研究的重要靶点。
1.Phenotype variations of retinal dystrophies caused by mutations in the RLBP1 gene., PMID:25429852
2.Comparative transcriptomic analysis provides insights into the molecular basis of the metamorphosis and nutrition metabolism change from zoeae to megalopae in Eriocheir sinensis., PMID:25460100
3.A transient wave of BMP signaling in the retina is necessary for Müller glial differentiation., PMID:25605781
4.CRALBP supports the mammalian retinal visual cycle and cone vision., PMID:25607845
5.AAV-mediated RLBP1 gene therapy improves the rate of dark adaptation in Rlbp1 knockout mice., PMID:26199951
6.Enriched Cultures of Retinal Cells From BJNhem20 Human Embryonic Stem Cell Line of Indian Origin., PMID:26567782
7.Microphthalmia-associated transcription factor regulates the visual cycle genes Rlbp1 and Rdh5 in the retinal pigment epithelium., PMID:26876013