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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHC44201 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHC44201 |
| 产品名称 | Recombinant Human ALOX5 Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P09917 |
| 蛋白长度 | Glu461-Ser671 |
| 预测分子量 | 26.70 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | 5-LO, ALOX5, LOG5, Polyunsaturated fatty acid 5-lipoxygenase, 5-lipoxygenase, Arachidonate 5-lipoxygenase |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
Q1:这款重组人 ALOX5 蛋白纯度如何?
A:经 SDS-PAGE 检测纯度大于 90%,可满足各类常规科研实验需求。
Q2:ALOX5 蛋白如何配制储备液?
A:直接使用无菌水溶解配置母液,具体操作可参照配套说明书。
Q3:储存方法与保质期?
A:经常取用可存放于 2~8℃;长期保存置于 - 20~-80℃,收货后保质期 12 个月。需使用手动除霜冰箱存放,避免反复冻融造成蛋白失活。
Q4:该蛋白适用哪些实验场景?
A:可用于 ELISA、免疫原制备、SDS-PAGE、WB 实验,生物活性还在验证阶段,仅可用于科研研究。
前沿相关研究:
1.Uremic toxin indoxyl sulfate induces trained immunity via the AhR-dependent arachidonic acid pathway in end-stage renal disease (ESRD)., PMID:38980302
2.CRISPR/Cas9-mediated neuronal deletion of 5-lipoxygenase alleviates deficits in mouse models of epilepsy., PMID:39048074
3.Quercetin Alleviates LPS-Stimulated Myocardial Injury through Regulating ALOX5/PI3K/AKT Pathway in Sepsis., PMID:39068603
4.Elucidating the role of S100A10 in CD8+ T cell exhaustion and HCC immune escape via the cPLA2 and 5-LOX axis., PMID:39117605
5.Radiation-Induced miRNAs Changes and cf mtDNA Level in Trauma Surgeons: Epigenetic and Molecular Biomarkers of X-ray Exposure., PMID:39126012
6.Oral administration of Robinia pseudoacacia L. flower exosome-like nanoparticles attenuates gastric and small intestinal mucosal ferroptosis caused by hypoxia through inhibiting HIF-1α- and HIF-2α-mediated lipid peroxidation., PMID:39134988
7.ALOX5 contributes to glioma progression by promoting 5-HETE-mediated immunosuppressive M2 polarization and PD-L1 expression of glioma-associated microglia/macrophages., PMID:39142719