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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHC10801 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHC10801 |
| 产品名称 | Recombinant Human TYMS Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P04818 |
| 蛋白长度 | Gln36-Val313 |
| 预测分子量 | 34.21 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 别名 | TS, Thymidylate synthase, TSase, TYMS |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
TYMS(胸苷酸合成酶)是核酸合成关键酶,催化脱氧尿苷酸转化为胸苷酸,支撑DNA合成与细胞分裂增殖,人体各类增殖细胞均有表达。蛋白表达异常会阻碍核酸复制,是细胞增殖调控、核酸代谢方向常用研究靶点。
Q1:TYMS 重组蛋白生产体系与纯度标准?
A:大肠杆菌表达,携带 N 端 His 标签,分子量 34.21 kDa,SDS-PAGE 检测纯度>90%。
Q2:冻干 TYMS 蛋白复溶方法?
A:无菌纯水溶解配制储备液,详细操作步骤参照随货数据表。
Q3:蛋白保存方法与时效?
A:频繁使用置于 2~8℃,长期分装放 - 20~-80℃,有效期 12 个月,规避反复冻融。
Q4:适用实验场景与使用限制?
A:可开展 WB、ELISA、免疫原制备实验,生物活性验证中,仅用于科研使用。
前沿相关研究:
1.The RNA-binding protein RRP1 brakes macrophage one-carbon metabolism to suppress autoinflammation.PMID: 40715096.
2.Pleckstrin-2 promotes the progression of colorectal cancer via YTHDF2-mediated TYMS mRNA stability. PMID: 40682666.
3.CAPRIN1/TYMS/MTHFD2 axis promotes EMT process in nasopharyngeal carcinoma development.PMID: 40246062.
4.Chidamide enhances the sensitivity of gastric cancer to 5-fluorouracil chemotherapy by suppressing the HDAC3/HNF4A/TYMS axis. PMID: 41326348.
5.Material basis revelation of anti-hepatoma effect of Huachansu (Cinobufacini) through down-regulation of thymidylate synthase. Chin Herb Med. PMID: 39949800.
6.TYMS Enhances Colorectal Cell Antioxidant Capacity Via the KEAP1-NRF2 Pathway to Resist Ferroptosis. PMID:39744483.
7.Integrated proteomic analysis identifies TYMS-dependent AMPK-mTOR signaling in pancreatic neuroendocrine tumors.PMID:41362763.