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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHC07001 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHC07001 |
| 产品名称 | Recombinant Human CD230/PRNP Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P04156 |
| 蛋白长度 | Lys23-Ser230 |
| 预测分子量 | 25.06 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. The highest reconstitution concentration is 0.2mg/ml. |
| 别名 | PRNP, PrP27-30, PRIP, PrP, CD230, ALTPRP, ASCR, PrP33-35C, PRP, Major prion protein |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
CD230/PRNP(朊蛋白)为细胞膜表面糖蛋白,参与细胞粘附、神经细胞存活调控,神经细胞广泛表达。蛋白构型异常会诱发蛋白聚集沉积,是神经细胞稳态、神经退行问题研究常用靶点。
Q1:CD230/PRNP 蛋白核心规格参数?
A:大肠杆菌表达、N 端 His 标签,分子量 25.06 kDa,经 SDS-PAGE 验证纯度>90%。
Q2:复溶有无浓度限制?
A:使用无菌水配制储备液,上限档位复溶浓度不可超过 0.2mg/mL,详细步骤查阅说明书。
Q3:如何储存维持蛋白稳定性?
A:频繁取用可 2~8℃放置,长期分装后置于 - 20~-80℃,有效期 12 个月,禁止反复冻融。
Q4:适用实验范围与使用规定?
A:可做 WB、ELISA、免疫原制备,活性尚在检测,仅允许科研场景使用。
前沿相关研究研究:
1.Three-colour flow cytometric detection of PrP in ovine leukocytes., PMID:17320973
2.Functional mechanisms of the cellular prion protein (PrP(C)) associated anti-HIV-1 properties., PMID:22076653
3.Cellular Prion Protein PrPC and Ecto-5'-Nucleotidase Are Markers of the Cellular Stress Response to Aneuploidy., PMID:28377454
4.Alveolar soft-part sarcoma (ASPS) resembles a mesenchymal stromal progenitor: evidence from meta-analysis of transcriptomic data., PMID:32596059
5.Increase in CD230 (cellular prion protein) fluorescence on blood lymphocytes in bovine spongiform encephalopathy-infected nonhuman primates., PMID:19843289