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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHC05201 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHC05201 |
| 产品名称 | Recombinant Human HMGCR Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in 20mM Tris, 20mM NaCl, 5% Trehalose, 2% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P04035 |
| 蛋白长度 | Glu441-Asn875 |
| 预测分子量 | 48.91 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | 3-hydroxy-3-methylglutaryl-coenzyme A reductase, HMGCR, HMG-CoA reductase |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
背景介绍:
HMGCR(羟甲基戊二酰辅酶A还原酶),胆固醇合成限速关键酶,主导体内胆固醇生成过程,在肝脏中大量表达。该蛋白活性异常会打乱脂质代谢水平,是脂代谢调控、胆固醇合成方向的经典研究靶点。
Q1: 这款重组人HMGCR蛋白的纯度和分子量是多少?
A: 该蛋白纯度大于90%,经SDS-PAGE验证;预测分子量为48.91 kDa,覆盖Glu441-Asn875区段,适用于ELISA、免疫原制备、Western Blot等实验。
Q2: 该蛋白的表达系统和标签是什么?
A: 该蛋白在大肠杆菌(E. coli)中表达,N端带有His标签(N-His),便于纯化和检测。
Q3: 这款蛋白的生物活性是否经过验证?
A: 目前生物活性测试正在进行中,该蛋白已确认可用于ELISA、免疫原、SDS-PAGE和WB等应用。
Q4: 如何正确复溶和储存该蛋白?
A: 蛋白为冻干粉形式,建议使用无菌水复溶。短期频繁使用可置于2-8°C保存,长期储存建议置于-20至-80°C,避免反复冻融。
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