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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHC03201 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHC03201 |
| 产品名称 | Recombinant Human ESR1/ER-alpha Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P03372 |
| 蛋白长度 | Met297-Ser554 |
| 预测分子量 | 31.78 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | Estrogen receptor, ER-alpha, ESR, ER, ESR1, Nuclear receptor subfamily 3 group A member 1, NR3A1, Estradiol receptor |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
ESR1(雌激素受体α)属于核受体家族,结合雌激素后进入细胞核调控基因转录,参与上皮生长、代谢调控、组织发育等生理活动,多组织均有分布。蛋白功能异常会造成激素调控失衡,是激素调控、细胞分化领域常用研究靶点。
Q1:这款重组人 ESR1/ER-α 蛋白纯度如何?
A:经 SDS-PAGE 检测纯度>90%,可满足各类常规科研实验需求。
Q2:ESR1 蛋白如何复溶配制储备液?
A:采用无菌水复溶配制储备液,详细操作方法可查阅随货说明书。
Q3:储存方式与保质期?
A:频繁取用可放置在 2~8℃保存;长期储存置于 - 20~-80℃环境,收货后保质期 12 个月。需使用手动除霜冰箱,避免反复冻融导致蛋白活性丧失。
Q4:重组人 ESR1 蛋白适用哪些实验?
A:可用于 ELISA、免疫原制备、SDS-PAGE、WB 实验,生物活性检测正在进行,only科研场景使用。
前沿相关研究:
1.Discovery and Characterization of PVTX-321 as a Potent and Orally Bioavailable Estrogen Receptor Degrader for ER+/HER2- Breast Cancer., PMID:40366756
2.ESR1 testing on FFPE samples from metastatic lesions in HR + /HER2- breast cancer after progression on CDK4/6 inhibitor therapy., PMID:40369610
3.Targeted Knockdown of Epithelial Estrogen Receptor α to Mitigate Ferroptosis and Epithelial-Mesenchymal Transition in Eosinophilic Asthma., PMID:40424612
4.Daidzein alleviates ethanol-induced acute gastric injury in rats by targeting ESR1 and activating the PI3K/AKT/CREB signaling pathway., PMID:404665035.
5.Unveiling pharmacological targets of Rihimaside C for radiation-induced lung injury: An in silico and experimental integrated approach., PMID:40486275
6.Integrating metabolomics and network pharmacology analysis provides new insights into the lipid metabolism disorder of Abrus mollis., PMID:40505757
7.Identification and validation of cellular senescence-related genes and immune cell infiltration characteristics in intervertebral disc degeneration., PMID:40510349