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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHC01501 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHC01501 |
| 产品名称 | Recombinant Human AFP Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | P02771 |
| 蛋白长度 | Leu260-Val609 |
| 预测分子量 | 41.44 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
AFP(甲胎蛋白)主要在胚胎阶段合成,成年阶段仅有微量表达。可参与细胞生长调控、物质转运等生理过程,机体表达量出现异常升高时,代表生理状态发生改变,常作为机体状态筛查的标志物,是发育生物学与生理筛查领域常用研究靶点。
Q1:这款重组人 AFP 蛋白的纯度是多少?
A:本产品经 SDS-PAGE 检测,纯度大于 90%,可满足各类常规科研实验需求。
Q2:该 AFP 蛋白如何复溶配制储备液?
A:可直接使用无菌水复溶配制储备液,参照产品配套说明书开展标准操作。
Q3:这款重组 AFP 蛋白的储存条件与保质期是什么?
A:日常频繁使用可保存于 2~8℃;长期保存需置于 - 20~-80℃环境,自收货之日起保质期 12 个月。储存需使用手动除霜冰箱,避免反复冻融,防止蛋白活性流失。
Q4:该重组人 AFP 蛋白可应用于哪些实验?
A:产品适用于 ELISA、免疫原制备、SDS-PAGE、WB 等实验,目前生物活性检测正在推进中,仅可用于科研用途。
前沿相关研究:
1.Melissa officinalis Attenuates Diclofenac-Induced Hepatorenal Toxicity via AMPK/SIRT1 and STAT3/Nrf2 Pathways., PMID:40294906
2.Combining AFP, PIVKA-II, and GP73 has diagnostic utility for hepatitis B-associated hepatocellular carcinoma and is consistent with liver pathology results., PMID:40295542
3.Integrated spatial and single cell transcriptomics identifies PRKDC as a dual prognostic biomarker and therapeutic target in hepatocellular carcinoma., PMID:40295654
4.Serum Golgi protein 73 (GP73) is a diagnostic and prognostic marker of hepatocellular carcinoma., PMID:40297156
5.The DNA Methylation is Involved in Liver Cancer Metastasis via Regulation of E- cadherin Gene., PMID:40302090
6.Alpha-fetoprotein and carbohydrate antigen 19-9 as prognostic biomarkers in acute liver failure: A retrospective study., PMID:40302660
7.The GALAD score performs better than AFP in hepatocellular carcinoma screening: a single-centre, case-control study in Malaysia., PMID:40303589