|

| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHB80401 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHB80401 |
| 产品名称 | Recombinant Human EFEMP2 Protein, N-GST |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 5% Trehalose, 2% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | O95967 |
| 蛋白长度 | Ser26-Phe443 |
| 预测分子量 | 73.69 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | EGF-containing fibulin-like extracellular matrix protein 2, Protein UPH1, Fibulin-4, FBLN4, EFEMP2, FIBL-4 |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
背景介绍:
EFEMP2(纤维蛋白 4)属于胞外基质糖蛋白,主导弹性纤维构筑、结缔组织发育,维系结缔组织与脉管结构完整,多类结缔组织细胞均可分泌。蛋白表达异常会破坏基质架构稳态,是胞外基质组装、结缔组织发育方向的常用研究靶点。
Q1: 这款重组人EFEMP2蛋白的基本参数是什么?
A1: 该蛋白在大肠杆菌(E. coli)中表达,N端带有GST标签(N-GST),覆盖Ser26-Phe443区段,预测分子量约73.69 kDa,纯度经SDS-PAGE验证大于90%。
Q2: 该蛋白可用于哪些实验应用?
A2: 目前验证可用于ELISA、免疫原制备、SDS-PAGE和Western Blot。生物活性测试正在进行中,如需latest数据请联系实验室。
Q3: 这款蛋白的内毒素水平是多少?
A3: 内毒素水平信息未在说明书中直接标注,如需具体检测数据,请联系实验室获取详细报告。
Q4: 收到冻干粉后如何正确处理和保存?
A4: 建议使用无菌水复溶。复溶后若频繁使用可置于2-8°C短期保存,长期储存建议分装后置于-20至-80°C,避免反复冻融,保质期为收货后12个月。
前沿相关研究:
1.Characterization of a spontaneous mouse model of mild, accelerated aging via ECM degradation in emphysematous lungs., PMID:37400563
2.EFEMP2 upregulates PD-L1 expression via EGFR/ERK1/2/c-Jun signaling to promote the invasion of ovarian cancer cells., PMID:37420173
3.miR-211-5p contributes to chondrocyte differentiation by suppressing Fibulin-4 expression to play a role in osteoarthritis., PMID:31396630
4.EFEMP2 increases the invasion ability of cervical cancer cells by promoting EMT via the Raf/MEK/ERK signaling pathway., PMID:36004647
5.Unraveling genetic threads: Identifying novel therapeutic targets for allergic rhinitis through Mendelian randomization., PMID:39040085
6.EFEMP2 suppresses epithelial-mesenchymal transition via Wnt/β-catenin signaling pathway in human bladder cancer., PMID:31592144