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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHB77701 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHB77701 |
| 产品名称 | Recombinant Human DDAH2 Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | O95865 |
| 蛋白长度 | Ala31-Leu265 |
| 预测分子量 | 27.10 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | DDAH, Dimethylargininase-2, NG30, DDAHII, DDAH2, Protein G6a, N(G),N(G)-dimethylarginine dimethylaminohydrolase 2, S-phase protein, G6A, Dimethylarginine dimethylaminohydrolase 2, DDAH-2 |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
DDAH2(二甲基精氨酸二甲胺水解酶2),代谢内源抑制因子、维持一氧化氮正常生成,调控内皮生理状态与微循环稳态,多表达于内皮、脏器实质细胞。蛋白表达紊乱会打破微循环平衡,是内皮稳态调控、微循环代谢研究常用靶点。
Q1:DDAH2 重组蛋白各项基础参数?
A:大肠杆菌表达、N-His 标签,理论分子量 27.10 kDa,经 SDS-PAGE 鉴定纯度大于 90%。
Q2:冻干粉末怎样配制母液?
A:采用无菌水进行复溶配制储备液,详细操作可查阅产品说明书。
Q3:如何储存保障蛋白活性稳定?
A:日常频繁使用可 2~8℃存放,长期置于 - 20~-80℃环境保存,有效期 12 个月,禁止反复冻融。
Q4:蛋白应用范围以及使用规定?
A:可用于 WB、ELISA、免疫原制备等实验,生物活性正在测试,仅可用于科研研究。
前沿相关研究:
1.Divergent Dimethylarginine Dimethylaminohydrolase Isoenzyme Expression in the Central Nervous System., PMID:34014421
2.Elevated Levels of ADMA Are Associated with Lower DDAH2 and Higher PRMT1 in LPS-Induced Endometritis Rats., PMID:29119338
3.Enhancing kidney DDAH-1 expression by adenovirus delivery reduces ADMA and ameliorates diabetic nephropathy., PMID:31904280
4.[Effects and related mechanism of 5-aza-2'-deoxycytidine on endothelial function in rats with hyperhomocysteinemia]., PMID:27545130
5.Determine exogenous human DDAH2 gene function in rabbit bone marrow-derived endothelial progenitor cells in vitro., PMID:28150318
6.Asymmetric dimethylarginine contributes to retinal neovascularization of diabetic retinopathy through EphrinB2 pathway., PMID:29777874