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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHB76001 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHB76001 |
| 产品名称 | Recombinant Human AIFM1/AIF Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Liquid |
| 储存缓冲液 | Supplied as a 0.2 μm filtered solution of PBS, 50% Glycerol, 2mM EDTA, 0.5M Argine, 5% Trehalose, pH 7.4. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | O95831 |
| 蛋白长度 | Leu102-Asp613 |
| 预测分子量 | 58.12 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | |
| 别名 | AIFM1, AIF, Programmed cell death protein 8, PDCD8, Apoptosis-inducing factor 1, mitochondrial |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
AIFM1(凋亡诱导因子)为线粒体功能蛋白,应激状态下释放入胞核,诱导染色质裂解、推动细胞程序性消亡,广泛表达于机体细胞。蛋白表达紊乱会扰乱细胞存活平衡,是线粒体凋亡通路、细胞命运调控方向常用研究靶点。
Q1:AIFM1 蛋白形态、表达系统与纯度如何?
A:大肠杆菌表达 N‑His 标签蛋白,液态形式,浓度 1.3 mg/mL,分子量 58.12 kDa,纯度>90%。
Q2:本品缓冲液组成有哪些?
A:pH7.4 PBS 体系,添加甘油、EDTA、精氨酸、海藻糖,经过 0.2μm 除菌过滤。
Q3:怎样储存可以长期保持稳定?
A:频繁取用 2~8℃存放,长期‑20~‑80℃保存,有效期 12 个月,避免反复冻融。
Q4:蛋白适用范围与使用要求?
A:可用于 WB、ELISA、免疫原制备,活性验证中,仅用于科研使用。
前沿相关研究:
1.AIF translocation into nucleus caused by Aifm1 R450Q mutation: generation and characterization of a mouse model for AUNX1., PMID:38449065
2.p20BAP31 induces cell apoptosis via both AIF caspase-independent and the ROS/JNK mitochondrial pathway in colorectal cancer., PMID:36977989
3.Ketamine induces insomnia-like symptom of zebrafish at environmentally relevant concentrations by mediating GABAergic synapse., PMID:38567678
4.Augmented mitochondrial apoptotic signaling impairs C2C12 myoblast differentiation following cellular aging through sequential passaging., PMID:38212955
5.Penehyclidine hydrochloride activates PARK2 and modulates ubiquitination of AIFM1 to rescue renal tubular injury in diabetic kidney disease., PMID:39828393
6.Impact of combined intermittent fasting and high-intensity interval training on apoptosis and atrophy signaling in rat fast- and slow-twitch muscles., PMID:39138135
7.Unlocking cardioprotection: iPSC exosomes deliver Nec-1 to target PARP1/AIFM1 axis, alleviating HF oxidative stress and mitochondrial dysfunction., PMID:39061056