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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHB72401 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHB72401 |
| 产品名称 | Recombinant Human CXCL14/BRAK/MIP-2G Protein, N-GST |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | O95715 |
| 蛋白长度 | Ser35-Glu111 |
| 预测分子量 | 36.25 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | SCYB14, CXCL14, C-X-C motif chemokine 14, Chemokine BRAK, MIP-2G, NJAC, Small-inducible cytokine B14, MIP2G |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
CXCL14/BRAK/MIP-2G属于CXC型趋化因子,招募单核细胞、树突状细胞定向迁移,参与免疫稳态调控,兼具调控脉管新生活性,成纤维细胞、上皮细胞均可分泌。表达失衡会扰乱组织免疫微环境,是免疫细胞趋化、组织微环境重塑方向常用研究靶点。
Q1:这款人源 CXCL14 蛋白采用什么表达平台,纯度是否稳定?
A:采用大肠杆菌表达系统制备,携带 N 端 GST 标签,理论分子量 36.25 kDa,经 SDS-PAGE 检测纯度稳定>90%。
Q2:收到冻干 CXCL14 蛋白后该怎样正确复溶使用?
A:建议使用无菌纯水复溶配制储备液,操作轻柔,避免剧烈震荡,更好保留蛋白特性。
Q3:样品如何存放防止失活,延长使用周期?
A:短期频繁使用可 2–8℃保存,长期建议分装后置于 - 20℃~-80℃低温储存,收货有效期 12 个月,务必避免反复冻融。
Q4:该蛋白可开展哪些科研实验,存在哪些使用限制?
A:适用于 WB、ELISA、抗体制备免疫原等实验,生物活性处于验证阶段,仅用于科学研究,不可应用于临床及人体相关试验。
前沿相关研究:
1.CXCL14 as a Key Regulator of Neuronal Development: Insights from Its Receptor and Multi-Omics Analysis., PMID:38338930
2.Characterizing the diabetes-induced pathological changes of the mouse lung by single-cell RNA sequencing., PMID:39832739
3.Decoding the cytokine code for heart failure based on bioinformatics, machine learning and Bayesian networks., PMID:39909085
4.Stat3 activation-triggered transcriptional networks govern the early stage of HBV-induced hepatic inflammation., PMID:38440978
5.Osteosarcoma Cells Secrete CXCL14 That Activates Integrin α11β1 on Fibroblasts to Form a Lung Metastatic Niche., PMID:38295227
6.PAX6/CXCL14 regulatory axis promotes the repair of corneal injury by enhancing corneal epithelial cell proliferation., PMID:38750454
7.Dual roles of HK3 in regulating the network between tumor cells and tumor-associated macrophages in neuroblastoma., PMID:38714539