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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHB43201 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHB43201 |
| 产品名称 | Recombinant Human MTA2 Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | O94776 |
| 蛋白长度 | Met1-Cys394 |
| 预测分子量 | 47.25 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 别名 | PID, Metastasis-associated 1-like 1, MTA2, p53 target protein in deacetylase complex, MTA1L1, Metastasis-associated protein MTA2, MTA1-L1 protein |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
背景介绍:
MTA2(转移相关蛋白2)属于核基质调控蛋白,参与染色质修饰,调控基因转录、细胞增殖迁移,广泛存在于机体各类细胞。表达异常会扰乱细胞生长平衡,是表观遗传调控、细胞侵袭机制方向常用研究靶点。
Q1:MTA2 重组蛋白核心参数是什么?
A:大肠杆菌表达搭配 N-His 标签,分子量 47.25 kDa,电泳检测纯度大于 90%。
Q2:冻干粉末该怎样复溶使用?
A:无菌纯水溶解配制储备液,完整操作方案详见附赠说明书。
Q3:正确保存方式与保质期?
A:频繁取用 2~8℃存放,长期 - 20~-80℃避光保存,有效期 12 个月,规避反复冻融。
Q4:蛋白适用实验以及使用范围?
A:可做 WB、ELISA、免疫原制备,活性正在验证,仅用于科研场景使用。
前沿相关研究:
1.New prognostic markers revealed by RNA-Seq transcriptome analysis after MYC silencing in a metastatic gastric cancer cell line., PMID:31645899
2.MTA2 as a Potential Biomarker and Its Involvement in Metastatic Progression of Human Renal Cancer by miR-133b Targeting MMP-9.,PMID:31771219
3.lncRNA PCAT1 might coordinate ZNF217 to promote CRC adhesion and invasion through regulating MTA2/MTA3/Snai1/E-cadherin signaling., PMID:35809308
4.Long Noncoding RNA TTC39A-AS1 Promotes Breast Cancer Tumorigenicity by Sponging MicroRNA-483-3p and Thereby Upregulating MTA2., PMID:34488224
5.Loss of MTA2-mediated downregulation of PTK7 inhibits hepatocellular carcinoma metastasis progression by modulating the FAK-MMP7 axis., PMID:38050825
6.The stoichiometry and interactome of the Nucleosome Remodeling and Deacetylase (NuRD) complex are conserved across multiple cell lines., PMID:30828972
7.An in vivo functional genomics screen of nuclear receptors and their co-regulators identifies FOXA1 as an essential gene in lung tumorigenesis., PMID:32512502
8.Loss of MTA2-mediated downregulation of PTK7 inhibits hepatocellular carcinoma metastasis progression by modulating the FAK-MMP7 axis., PMID:38050825