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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHB40801 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHB40801 |
| 产品名称 | Recombinant Human DFFB Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | B4DZS0 |
| 蛋白长度 | Met1-Gln362 |
| 预测分子量 | 44.27 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | Caspase-activated nuclease, DFF2, Caspase-activated DNase, CAD, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, DFF40, DFFB, DFF-40, CPAN |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
DFFB(DNA片段化因子β亚基)为凋亡效应蛋白,活化后降解基因组DNA,推动细胞程序性消亡,各类体细胞普遍存在。蛋白异常会干扰细胞凋亡进程,是细胞程序性消亡、细胞命运调控方向常用研究靶点。
Q1:重组 DFFB 蛋白表达体系与纯度规格?
A:大肠杆菌表达,N 端 His 标签,分子量 44.27 kDa,SDS-PAGE 纯度>90%。
Q2:冻干蛋白复溶方案是什么?
A:采用无菌纯水溶解配置储备液,详细操作参照配套说明书。
Q3:储存方法以及保质期多久?
A:频繁使用 2~8℃存放,长期 - 20~-80℃保存,有效期 12 个月,禁止反复冻融。
Q4:蛋白用途与使用规范?
A:可用于 WB、ELISA、制免疫原等实验,活性尚在验证,仅用于科研使用。
前沿相关研究:
1.Multi-CpG linear regression models to accurately predict paclitaxel and docetaxel activity in cancer cell lines., PMID:36990534
2.Early apoptotic reorganization of spliceosomal proteins involves caspases, CAD and rearrangement of NuMA., PMID:22023725
3.A proteomic analysis of p53-independent induction of apoptosis by bortezomib in 4T1 breast cancer cell line., PMID:25305590
4.Sensitization of breast cancer cells to doxorubicin via stable cell line generation and overexpression of DFF40., PMID:26529233
5.Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes., PMID:30799275
6.Genomic origin, fragmentomics, and transcriptional properties of long cell-free DNA molecules in human plasma., PMID:38408788
7.Cheating death at the dawn of life: developmental control of apoptotic repression in the preimplantation embryo., PMID:21888895
8.Human papillomavirus type 16 E6 protein inhibits DNA fragmentation via interaction with DNA fragmentation factor 40., PMID:22609799