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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHB04001 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHB04001 |
| 产品名称 | Recombinant Human SMPD2 Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | O60906 |
| 蛋白长度 | Met1-Arg236 |
| 预测分子量 | 29.66 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | Neutral sphingomyelinase, Lyso-PAF-PLC, SMPD2, Sphingomyelin phosphodiesterase 2, nSMase, Lyso-platelet-activating factor-phospholipase C, nSMase1, N-SMase |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
SMPD2(中性鞘磷脂酶2)属于鞘脂代谢关键水解酶,催化鞘磷脂分解生成神经酰胺,介导胞内信号传递,调控细胞增殖、程序性凋亡,广泛分布于各类体细胞内质网与胞核。蛋白活性异常会打乱鞘脂代谢平衡,是鞘脂代谢通路、细胞生命进程调控方向常用研究靶点。
Q1:该 SMPD2 蛋白采用何种制备方式,纯度表现如何?
A:原核大肠杆菌表达系统构建 N 端 His 标签重组蛋白,理论分子量 29.66 kDa,经电泳检测纯度高于 90%。
Q2:蛋白复溶过程有无注意事项?
A:使用无菌纯水溶解配制成母液即可,完整复溶配比与操作细节可查阅随货说明书。
Q3:储存方式和保质期?
A:日常 2~8℃存放,长期 - 20~-80℃保存,有效期 12 个月,避免反复冻融。
Q4:该蛋白可以做哪些实验,能否用于临床?
A:支持 WB、ELISA、免疫抗原制备等实验,生物活性仍在验证,仅允许科研实验,不可用于临床。
前沿相关研究:
1.Identifying rare variants for genetic risk through a combined pedigree and phenotype approach: application to suicide and asthma., PMID:25335167
2.Induction of membrane ceramides: a novel strategy to interfere with T lymphocyte cytoskeletal reorganisation in viral immunosuppression., PMID:19834551
3.Bioeffects of ultrasound-stimulated microbubbles on endothelial cells: gene expression changes associated with radiation enhancement in vitro., PMID:22980406
4.Modulation of Ceramide-Induced Apoptosis in Enteric Neurons by Aryl Hydrocarbon Receptor Signaling: Unveiling a New Pathway beyond ER Stress., PMID:39201268
5.The Effects and Mechanism of Scutellaria baicalensis Georgi Stems and Leaves Flavonoids on Myelin Sheath Degeneration Induced by Composite Aβ in Rats., PMID:37218194
6.Transcriptional regulation of neutral sphingomyelinase 2 gene expression of a human breast cancer cell line, MCF-7, induced by the anti-cancer drug, daunorubicin., PMID:19698806