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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHA93001 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHA93001 |
| 产品名称 | Recombinant Human ADCY9 Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | O60503 |
| 蛋白长度 | Asp1029-Val1282 |
| 预测分子量 | 31.11 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 别名 | ADCY9, Adenylate cyclase type 9, AC9, ATP pyrophosphate-lyase 9, Adenylate cyclase type IX, ACIX, KIAA0520, Adenylyl cyclase 9 |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
ADCY9(腺苷酸环化酶 9)属于跨膜信号合成酶,催化生成胞内信使 cAMP,介导胞内信号转导,调控细胞增殖与功能活化,广泛分布于多种组织细胞。表达失衡会扰乱胞内信号传导网络,是 cAMP 信号通路、细胞功能调控方向常用研究靶点。
Q1:这款人源 ADCY9 蛋白采用什么表达平台,纯度是否稳定?
A:采用大肠杆菌表达系统制备,携带 N 端 His 标签,理论分子量 31.11 kDa,经 SDS-PAGE 检测纯度稳定>90%。
Q2:收到冻干 ADCY9 蛋白后该怎样正确复溶使用?
A:建议使用无菌纯水复溶配制储备液,操作轻柔,避免剧烈震荡,详细操作可参考随货数据表。
Q3:样品如何存放防止失活,延长使用周期?
A:短期频繁使用可 2–8℃保存,长期建议分装后置于 - 20℃~-80℃低温储存,收货有效期 12 个月,务必避免反复冻融。
Q4:该蛋白可开展哪些科研实验,存在哪些使用限制?
A:适用于 WB、ELISA、抗体制备免疫原等实验,生物活性处于验证阶段,仅用于科学研究,不可应用于临床及人体相关试验。
前沿相关研究:
1.Genetic polymorphisms of regulatory T cell-related genes modulate systemic inflammation induced by viral hepatitis., PMID:34166564
2.Effect of Soluble Adenylyl Cyclase (ADCY10) Inhibitors on the LH-Stimulated cAMP Synthesis in Mltc-1 Leydig Cell Line., PMID:33924969
3.Delayed KCNQ1/KCNE1 assembly on the cell surface helps IKs fulfil its function as a repolarization reserve in the heart., PMID:33963564
4.Meta-Analysis Identifies BDNF and Novel Common Genes Differently Altered in Cross-Species Models of Rett Syndrome., PMID:36232428
5.Elevated PDE4C level serves as a candidate diagnostic biomarker and correlates with poor survival in thyroid carcinoma., PMID:38514754
6.Targeting the Hippo and Rap1 signaling pathways: the anti-proliferative effects of curcumin in colorectal cancer cell lines., PMID:39779534
7.Identification and Characterization of Biomarkers and Their Role in Opioid Addiction by Integrated Bioinformatics Analysis., PMID:33328875