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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHA88001 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHA88001 |
| 产品名称 | Recombinant Human OPA1 Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >80% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | O60313 |
| 蛋白长度 | Ser607-Lys960 |
| 预测分子量 | 44.50 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | KIAA0567, OPA1, Optic atrophy protein 1, Dynamin-like 120 kDa protein, mitochondrial |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
OPA1(线粒体融合蛋白1)属于线粒体内膜GTP酶,管控内膜融合、线粒体嵴结构完整,保障产能代谢并抑制细胞凋亡,全身组织广泛表达。蛋白异常会造成线粒体结构紊乱、产能下降,是线粒体动力学、细胞能量稳态方向常用研究靶点。
Q1:重组人 OPA1 蛋白纯度如何?
A:经 SDS-PAGE 检测纯度>80%,可用于常规科研实验。
Q2:OPA1 蛋白如何配制储备液?
A:无菌水复溶配制母液,详细操作参照配套说明书。
Q3:储存要求与保质期?
A:频繁取用置于 2~8℃保存;长期 - 20~-80℃存放,收货有效期 12 个月。采用手动除霜冰箱储存,杜绝反复冻融,避免蛋白失活。
Q4:该蛋白适用哪些实验?
A:可开展 ELISA、免疫原制备、SDS-PAGE、WB 实验,生物活性正在测试,仅用于科研使用。
前沿相关研究:
1.Clioquinol induces mitochondrial toxicity in SH-SY5Y neuroblastoma cells by affecting the respiratory chain complex IV and OPA1 dynamin-like GTPase., PMID:40125820
2.ROS-DRP1-mediated excessive mitochondrial fission and autophagic flux inhibition contribute to heat stress-induced apoptosis in goat Sertoli cells., PMID:40234984
3.Leptin attenuates diabetic cardiomyopathy-induced cardiac remodeling via regulating cGAS/STING signaling and Opa1-mediated mitochondrial fusion., PMID:40246132
4.Soluble Epoxide Hydrolase Inhibition Improves Alzheimer's Disease Hallmarks: Correlation with Peripheral Inflammation and Gut Microbiota Modulation., PMID:40423637
5.mTOR inhibition triggers mitochondrial fragmentation in cardiomyocytes through proteosome-dependent prohibitin degradation and OPA-1 cleavage., PMID:40450326
6.1,25(OH)2D3 up-regulated mitochondrial dynamics and biogenesis to modulate steroidogenesis in the scent glands of muskrats (Ondatra zibethicus)., PMID:40398522