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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHA75501 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHA75501 |
| 产品名称 | Recombinant Human ACTN4 Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH7.4,1mM EDTA, 4%trehalose,1% mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | O43707 |
| 蛋白长度 | Tyr611-Leu911 |
| 预测分子量 | 36.79 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | Non-muscle alpha-actinin 4, Alpha-actinin-4, ACTN4 |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
ACTN4（α-辅肌动蛋白4）属于非肌型骨架蛋白，交联肌动蛋白纤维，稳固细胞骨架结构，管控细胞黏附、迁移与泌尿过滤单元足细胞形态完整，广泛分布于多种组织细胞。蛋白表达异常会破坏细胞架构稳态，是细胞骨架组装、细胞迁移调控方向常用研究靶点。
常见问题（FAQs）：
Q1: 这款重组人ACTN4蛋白的纯度和分子量是多少？
A: 该蛋白纯度大于90%，经SDS-PAGE验证；预测分子量为36.79 kDa，覆盖Tyr611-Leu911区段，适用于ELISA、免疫原制备、Western Blot等实验。
Q2: 该蛋白的表达系统和标签是什么？
A: 该蛋白在大肠杆菌（E. coli）中表达，N端带有His标签（N-His），便于纯化和检测。
Q3: 这款蛋白的生物活性是否经过验证？
A: 目前生物活性测试正在进行中，该蛋白已确认可用于ELISA、免疫原、SDS-PAGE和WB等应用。内毒素水平信息请联系实验室获取。
Q4: 如何正确复溶和储存该蛋白？
A: 蛋白为冻干粉形式，建议使用无菌水复溶。短期频繁使用可置于2-8°C保存，长期储存建议置于-20至-80°C，避免反复冻融。
1.m6A modification of lncRNA PHKA1-AS1 enhances Actinin Alpha 4 stability and promotes non-small cell lung cancer metastasis., PMID:38764726
2.The Immunosuppressive Drug LF15-0195 Acts Also on Glomerular Lesions, by a Change in Cytoskeleton Distribution in Podocyte., PMID:39074452
3.Live-cell imaging and CLEM reveal the existence of ACTN4-dependent ruffle-edge lamellipodia acting as a novel mode of cell migration., PMID:39222868
4.Identification of multicohort-based predictive signature for NMIBC recurrence reveals SDCBP as a novel oncogene in bladder cancer., PMID:39873429
5.DANCR knockdown alleviates neuroinflammation and functional recovery after spinal cord injury via regulating the ACTN4 / STAT3 axis., PMID:39798643
6.PHF23 promotes NSCLC proliferation, metastasis, and chemoresistance via stabilization of ACTN4 and activation of the ERK pathway., PMID:37626047
