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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHA59501 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHA59501 |
| 产品名称 | Recombinant Human CYP26A1 Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | O43174 |
| 蛋白长度 | Arg73-Ile255 |
| 预测分子量 | 23.23 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | P450RAI1, Cytochrome P450 26A1, Cytochrome P450RAI, hP450RAI, CYP26A1, Cytochrome P450 retinoic acid-inactivating 1, Retinoic acid-metabolizing cytochrome, CYP26, Retinoic acid 4-hydroxylase |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
CYP26A1(细胞色素 P450 26A1)属于细胞色素 P450 氧化酶家族,介导视黄酸分解代谢,精细调控细胞分化与组织发育,多种上皮细胞均可表达。表达失衡会干扰视黄酸信号水平,是维 A 酸代谢、细胞分化调控方向常用研究靶点。
Q1:这款人源 CYP26A1 蛋白采用什么表达平台,纯度是否稳定?
A:采用大肠杆菌表达系统制备,携带 N 端 His 标签,理论分子量 23.23 kDa,经 SDS-PAGE 检测纯度稳定>90%。
Q2:收到冻干 CYP26A1 蛋白后该怎样正确复溶使用?
A:建议使用无菌纯水复溶配制储备液,操作轻柔,避免剧烈震荡,更好保留蛋白特性。
Q3:样品如何存放防止失活,延长使用周期?
A:短期频繁使用可 2–8℃保存,长期建议分装后置于 - 20℃~-80℃低温储存,收货有效期 12 个月,务必避免反复冻融。
Q4:该蛋白可开展哪些科研实验,存在哪些使用限制?
A:适用于 WB、ELISA、抗体制备免疫原等实验,生物活性处于验证阶段,仅用于科学研究,不可应用于临床及人体相关试验。
前沿相关研究:
1.Identification of an inflammation-related risk signature for prognosis and immunotherapeutic response prediction in bladder cancer., PMID:38216619
2.TOP2B Is Required to Maintain the Adrenergic Neural Phenotype and for ATRA-Induced Differentiation of SH-SY5Y Neuroblastoma Cells., PMID:35831557
3.All-trans Retinoic Acid has Limited Therapeutic Effects on Cognition and Hippocampal Protein Expression After Controlled Cortical Impact., PMID:35878718
4.Hepatocyte Nuclear Factor 4α (HNF4α) Plays a Controlling Role in Expression of the Retinoic Acid Receptor β (RARβ) Gene in Hepatocytes., PMID:37239961
5.Mechanism of thyroid hormone and its structurally similar contaminant bisphenol S exposure on retinoid metabolism in zebrafish larval eyes., PMID:37748373
6.Distinctive Metabolism-Associated Gene Clusters That Are Also Prognostic in Intrahepatic Cholangiocarcinoma and Hepatocellular Carcinoma., PMID:36199423