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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHA51601 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHA51601 |
| 产品名称 | Recombinant Human NCAM2 Protein, N-GST |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | O15394 |
| 蛋白长度 | Leu21-Gln297 |
| 预测分子量 | 57.74 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | NCAM-2, N-CAM-2, NCAM21, NCAM2, Neural cell adhesion molecule 2 |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
NCAM2(神经细胞黏附分子2)属于免疫球蛋白超家族跨膜蛋白,介导细胞间识别与黏附,参与神经突触发育与神经元迁移,主要在神经组织表达。表达失衡会干扰神经细胞相互作用,是神经发育、细胞黏附调控方向常用研究靶点。
Q1:这款人源 NCAM2 蛋白采用什么表达平台,纯度是否稳定?
A:采用大肠杆菌表达系统制备,携带 N 端 GST 标签,理论分子量 57.74 kDa,经 SDS-PAGE 检测纯度稳定>90%。
Q2:收到冻干 NCAM2 蛋白后该怎样正确复溶使用?
A:建议使用无菌纯水复溶配制储备液,操作轻柔,避免剧烈震荡,更好保留蛋白特性。
Q3:样品如何存放防止失活,延长使用周期?
A:短期频繁使用可 2–8℃保存,长期建议分装后置于 - 20℃~-80℃低温储存,收货有效期 12 个月,务必避免反复冻融。
Q4:该蛋白可开展哪些科研实验,存在哪些使用限制?
A:适用于 WB、ELISA、抗体制备免疫原等实验,生物活性处于验证阶段,仅用于科学研究,不可应用于临床及人体相关试验。
前沿相关研究:
1.The polysialyltransferases interact with sequences in two domains of the neural cell adhesion molecule to allow its polysialylation., PMID:23341449
2.Identifying rare variants for genetic risk through a combined pedigree and phenotype approach: application to suicide and asthma., PMID:25335167
3.circ-Ncam2 (mmu_circ_0006413) Participates in LPS-Induced Microglia Activation and Neuronal Apoptosis via the TLR4/NF-κB Pathway., PMID:35687299
4.Using the Allen gene expression atlas of the adult mouse brain to gain further insight into the physiological significance of TAG-1/Contactin-2., PMID:32601750
5.Neural cell adhesion molecule 2 promotes the formation of filopodia and neurite branching by inducing submembrane increases in Ca2+ levels., PMID:25632147
6.Synaptic cell adhesion molecule-2 and collapsin response mediator protein-2 are novel members of the matrix metalloproteinase-9 degradome., PMID:22694054