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| 产地 | France |
| 品牌 | AntibodySystem |
| 货号 | YHA39001 |
| 用途 | 仅用于科研 |
| 包装规格 | 100 μg |
| 纯度 | 0.95% |
| CAS编号 | |
| 是否进口 | 否 |
| 货号 | YHA39001 |
| 产品名称 | Recombinant Human SEMA3E Protein, N-His |
| 规格 | 100 μg |
| 价格 | 1880 |
| 状态 | Lyophilized |
| 储存缓冲液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession | O15041 |
| 蛋白长度 | Arg32-Phe516 |
| 预测分子量 | 57.72 kDa |
| 稳定性与储存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 重悬 | Reconstitute in sterile water for a stock solution. |
| 别名 | SEMAH, Semaphorin-3E, SEMA3E, KIAA0331 |
| 物种 | Homo sapiens (Human) |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 备注 | For research use only. |
SEMA3E(信号素3E)属于分泌型信号素家族蛋白,介导细胞趋化与迁移调控,参与组织脉管构建与细胞通讯,多种组织细胞能够合成。表达失衡会干扰细胞定向移动,是细胞迁移调控、脉管形成机制方向常用研究靶点。
Q1:这款人源 SEMA3E 蛋白采用什么表达平台,纯度是否稳定?
A:采用大肠杆菌表达系统制备,携带 N 端 His 标签,理论分子量 57.72 kDa,经 SDS-PAGE 检测纯度稳定>90%。
Q2:收到冻干 SEMA3E 蛋白后该怎样正确复溶使用?
A:建议使用无菌纯水复溶配制储备液,操作轻柔,避免剧烈震荡,更好保留蛋白特性。
Q3:样品如何存放防止失活,延长使用周期?
A:短期频繁使用可 2–8℃保存,长期建议分装后置于 - 20℃~-80℃低温储存,收货有效期 12 个月,务必避免反复冻融。
Q4:该蛋白可开展哪些科研实验,存在哪些使用限制?
A:适用于 WB、ELISA、抗体制备免疫原等实验,生物活性处于验证阶段,仅用于科学研究,不可应用于临床及人体相关试验。
前沿相关研究:
1.The Inflammatory Gene PYCARD of the Entorhinal Cortex as an Early Diagnostic Target for Alzheimer's Disease., PMID:36672701
2.Platelet-instructed SPP1+ macrophages drive myofibroblast activation in fibrosis in a CXCL4-dependent manner., PMID:36807143
3.Sema-3E/PlexinD1 axis modulates dendritic cell phenotypes and functions: Current status and future implications., PMID:38772051
4.Semaphorin 3s signaling in the central nervous system: Mechanisms and therapeutic implication for brain diseases., PMID:39855276
5.A Novel Loss-of-Function SEMA3E Mutation in a Patient with Severe Intellectual Disability and Cognitive Regression., PMID:35628442
6.Plexin D1 negatively regulates macrophage-derived foam cell migration via the focal adhesion kinase/Paxillin pathway., PMID:38897039
4.Vascular Sema3E-Plexin-D1 Signaling Reactivation Promotes Post-stroke Recovery through VEGF Downregulation in Mice., PMID:33978913
5.Constraint-Based, Score-Based and Hybrid Algorithms to Construct Bayesian Gene Networks in the Bovine Transcriptome., PMID:35625151