Tags: 抗Toledo whole cell lysate(细胞裂解液) supplier,抗Toledo whole cell lysate(细胞裂解液) purchase,抗Toledo whole cell lysate(细胞裂解液) manufacturer,抗Toledo whole cell lysate(细胞裂解液) distributor,抗Toledo whole cell lysate(细胞裂解液) cost,抗Toledo whole cell lysate(细胞裂解液) buy,抗Toledo whole cell lysate(细胞裂解液) for sale

Toledo whole cell lysate(细胞裂解液)

Catalog No.

CLH00125

Form

Liquid

Storage buffer

1 X Sample Buffer (1X RIPA buffer + 1X SDS loading buffer).

Concentration

100 μg at 2 mg/ml

Applications

WB

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at -20 to -80 °C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Aiming to develop the most reliable bioreagents, AntibodySystem SAS was founded in 2019 in France by a team of professionals from top industrial companies and research institutes. The collective scientific and technical experiences in our team have brought breaking throughs in multiple product developments, including recombinant antibodies, recombinant proteins, biosimilar, RUO ELISA kits and other related products. By working with talented researchers and technicians, AntibodySystem continues to upgrade product qualities and lower the overall cost, offering accountable and affordable solutions for biolabs all over the world. Since its establishment, AntibodySystem has built long term and stable collaborations with academia, pharmaceutical industry, biotech companies and research hospitals, supporting their R&D by AntibodySystem's bioreagents with excellent data accuracy and reproducibility.

AntibodySystem由具有30多年蛋白抗体开发经验的 创立于法国,专注于生命科学和生物制药领域研究,***。AntibodySystem自主开发了高效、高产的真核重组表达系统,利用该系统生产了高质量的重组蛋白、抗体产品。目前产品包括药物靶点蛋白以及对照抗体药物、Invivo功能性抗体、SAA系列流式抗体、PEG抗体、磷酸化抗体、抗小分子抗体、ADA抗体、PK&ADA ELISA试剂盒。


佰乐博生物作为AntibodySystem在亚洲区授权 ,致力于为广大科研工作者提供 的产品服务。

如果您对我们的产品感兴趣,请联系027-65279366。

以上产品仅用于科研