一、中文名称：IdeS Protease (IgG specific) (YXX05001),免疫球蛋白特异性IdeS蛋白酶
二、产品描述
ldeS Protease全称免疫球蛋白G降解酶(lmmunoglubulin G-degrading enzyme ofStreptococcus pyogenes,ldeS)，是由人类致病菌酿脓链球菌（Streptococcuspyogenes）产生并分泌至胞外的一种半胱氨酸水解酶。该蛋白酶具有极高的底物特异性，仅能识别lgG，在抗体下较链区的特定位点进行酶切，使lgG水解为完整的F(ab')2片段和Fc片段。ldeS可识别人源和其他多种动物来源的lgG，比如鼠、兔、猴、羊以及人动物嵌合lgG等。ldeS具有独特的底物选择性，可以作为工具酶应用于抗体类药物或抗体融合蛋白药物的结构表征分析。本产品利用大肠杆菌重组表达，纯度高，具有良好的酶切活性，是用于表征 性抗体、Fc融合蛋白和抗体-药物复合物的有价值的工具。
三、产品详情
名称：IdeS Protease (IgG specific) (YXX05001)
货号：YXX05001
生物活性：One unit will cleave ≥95% of 1μg of recombinant monoclonal IgG in 30 minutes at 37°C.
表达系统：E. coli
种属：Streptococcus pyogenes
蛋白长度：IdeS Protease is cloned from Streptococcus pyogenes and expressed in E.coli.
预测分子量：37.57 kDa
性质：Recombinant
靶标：IdeS Protease (IgG Specific)
浓度：40000U/ml
内毒素水平：Please contact with the lab for this information.
纯度：>90% as determined by SDS-PAGE.
应用：Proteomics
状态：Liquid
保存溶液：0.01M PBS, pH 7.4, 50% glycerol
实验操作流程：Digestion reaction condition: 37℃, 30min. Protocol: 1. Add appropriate amount of IgG (to 5mg) in digestive juice; 2. Add IdeS protease to IgG samples: add 1 unit of IdeS per 1ug of IgG; 3. Incubate the sample at 37°C for 30-60min. * IdeS proteases are most active in buffers at or near neutral pH. The recommended reaction buffer is 50 mM sodium phosphate and 150 mM NaCl (pH 6.6), but most common biological buffers are suitable, such as Tris or PBS. Buffers outside this pH range (such as acetate buffer) may also be suitable, but the incubation time or enzyme amount needs to be optimized according to the actual situation.
运输：In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储：Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 °C for one week. Store at -20 to -80 °C for twelve months from the date of receipt.
四、参考文献：
IdeS, a secreted proteinase of Streptococcus pyogenes, is bound to a nuclease at the bacterial surface where it inactivates opsonizing IgG antibodies.PMID:37838172
Anti-glomerular basement membrane disease (Goodpasture disease): From pathogenesis to plasma exchange to IdeS.PMID:34339589
Recombinant Endopeptidases IdeS and IdeZ and Their Potential Application.PMID:37748870
Streptococcal IdeS and its impact on immune response and inflammation.PMID:22248585
IdeS and SpeB: immunoglobulin-degrading cysteine proteinases of Streptococcus pyogenes.PMID:12615219
Polypept(o)ides: Hybrid Systems Based on Polypeptides and Polypeptoids.PMID:26398770
Toward Clinical use of the IgG Specific Enzymes IdeS and EndoS against Antibody-Mediated Diseases.PMID:27914091
The ides of MARCH5: The E3 ligase essential for peroxisome degradation by pexophagy.PMID:34889952
Current Trends in MOF (Metal-Organic Framework) and Metal X-ides.PMID:37446366
Rapid and improved characterization of therapeutic antibodies and antibody related products using IdeS digestion and subunit analysis.PMID:27156477
IgG-cleaving endopeptidase enables in vivo gene therapy in the presence of anti-AAV neutralizing antibodies.PMID:32483358
IdeS in anti-glomerular basement membrane disease: Is this the new deal?PMID:31648697
Transplantation: IdeS to desensitize organ allograft recipients.PMID:28890539
Streptococcal IdeS: therapeutic potential for Guillain-Barre syndrome.PMID:26194472
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