一、中文名称：Anti-HRSV F/Fusion glycoprotein F0 Antibody (Am14) (RVV02801)， Am14单克隆抗体
二、产品描述
人类呼吸道合胞病毒（HRSV）的融合糖蛋白（F0/F蛋白）在病毒感染细胞过程中扮演着关键角色。它属于I类病毒融合蛋白，负责介导病毒与宿主细胞膜之间的融合过程，从而使病毒基因组能够进入宿主细胞内。
结构和功能：
前体形式（F0）：F蛋白最初合成时为一个未剪切的前体形式F0，随后在宿主体内被宿主细胞的蛋白酶切割成两个亚单位，即F1和F2。
成熟形式（F）：成熟的F蛋白由F1和F2通过二硫键连接组成，并且含有一个融合肽段，该肽段插入宿主细胞膜中。F1亚单位主要负责执行膜融合的功能，而F2则帮助稳定蛋白质结构。
激活机制：
F蛋白需要经历一系列构象变化才能从预融合状态转变为后融合状态，在这个过程中完成病毒包膜与宿主细胞膜的融合。
研究进展：
关于F蛋白的研究揭示了其在病毒侵入过程中的重要性，并且是疫苗开发和抗病毒药物设计的关键靶点。例如：
抗体识别位点：研究发现某些单克隆抗体可以特异性地结合到F蛋白的预融合形态上的特定表位，阻止病毒与宿主细胞的融合，从而抑制感染。
疫苗设计：基于F蛋白的结构信息，科学家们正在尝试设计更有效的疫苗，旨在诱导产生针对F蛋白的有效免疫反应，特别是针对其预融合构象的抗体反应。
F蛋白不仅是理解HRSV如何感染宿主细胞的基础，也是开发新的治疗方法的重要目标。
三、产品详情
名称：Anti-HRSV F/Fusion glycoprotein F0 Antibody (Am14) (RVV02801)
别名：Am14，单克隆抗体，F,Fusion glycoprotein F0, Human Respiratory Syncytial Virus (HRSV),RSV,HMPV,Human Metapneumovirus (hMPV)
货号：RVV02801
表达系统：Mammalian Cells
种属反应性：HRSV-A
宿主：Human
同种型：IgG1
克隆类型：Monoclonal
经测试应用：ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:5000
靶标：F, Fusion glycoprotein F0
浓度：1.67 mg/ml
内毒素水平：Please contact with the lab for this information.
纯度：>95% as determined by SDS-PAGE.
纯化方式：Protein A/G purified from cell culture supernatant.
Accession号：P03420
克隆号：Am14
应用：ELISA, IHC, WB
状态：Liquid
保存溶液：0.01M PBS, pH 7.4.
稳定性和存储：Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
四、参考文献：
Infectivity of a human respiratory syncytial virus lacking the SH, G, and F proteins is efficiently mediated by the vesicular stomatitis virus G protein. PMID: 12610153
Identification of subgroups of bovine respiratory syncytial virus. PMID: 1583108
Structures of Langya Virus Fusion Protein Ectodomain in Pre- and Postfusion Conformation. PMID: 37278642
Transmembrane Domain Dissociation Is Required for Hendra Virus F Protein Fusogenic Activity. PMID: 31462574
Development of liposomes and pseudovirions with fusion activity for efficient gene delivery. PMID: 22023472
Molecular Features of the Measles Virus Viral Fusion Complex That Favor Infection and Spread in the Brain. PMID: 34061592
A Randomized Control Trial Comparing Local Autografts and Allografts in Single Level Anterior Cervical Discectomy and Fusion Using a Stand-Alone Cage. PMID: 33189111
Mutations in the parainfluenza virus 5 fusion protein reveal domains important for fusion triggering and metastability. PMID: 24089572
Triggering of the newcastle disease virus fusion protein by a chimeric attachment protein that binds to Nipah virus receptors. PMID: 21460213
Identification of residues in the human respiratory syncytial virus fusion protein that modulate fusion activity and pathogenesis. PMID: 25339762
Respiratory Syncytial Virus Attachment Glycoprotein Contribution to Infection Depends on the Specific Fusion Protein. PMID: 26468535
The electrophysiological net response ('F-complex') to spatial fusion of speech elements forming an auditory object. PMID: 12738428
Ephrin-B2 and ephrin-B3 as functional henipavirus receptors. PMID: 22227101
MRI T2-Weighted Imaging and Fat-Suppressed T2-Weighted Imaging Image Fusion Technology Improves Image Discriminability for the Evaluation of Anal Fistulas. PMID: 30799574
High-yield membrane protein expression from E. coli using an engineered outer membrane protein F fusion. PMID: 23345122
Does fusion to T2 compared with T3/T4 lead to improved shoulder balance in adolescent idiopathic scoliosis with a double thoracic curve? PMID: 29851712
Cytogenetic mapping of the Muller F element genes in Drosophila willistoni group. PMID: 25134938
Proteolytic activation of respiratory syncytial virus fusion protein. Cleavage at two furin consensus sequences. PMID: 11418598
Low-pH triggering of human metapneumovirus fusion: essential residues and importance in entry. PMID: 19036821
Construction of Recombinant Rabies Virus Vectors Expressing H or F Protein of Peste des Petits Ruminants Virus. PMID: 36288168
The 'F-complex' and MMN tap different aspects of deviance. PMID: 15661112
Studies on the fusion peptide of a paramyxovirus fusion glycoprotein: roles of conserved residues in cell fusion. PMID: 1548771
A cellular Drosophila melanogaster protein with similarity to baculovirus F envelope fusion proteins. PMID: 15956544
Can C3 Laminectomy Reduce Interlaminar Bony Fusion and Preserve the Range of Motion After Cervical Laminoplasty? PMID: 27517513
Genetic diversity and evolution of human metapneumovirus fusion protein over twenty years. PMID: 19740442
Cystic fibrosis transmembrane conductance regulator activation stimulates endosome fusion in vivo. PMID: 8901608
Meeting report VLPNPV: Session 3: Immune responses. PMID: 25529229
Genetics of Bladder Malignant Tumors in Childhood. PMID: 27013922
Novel pneumoviruses (PnVs): Evolution and inflammatory pathology. PMID: 23763766
Virus-like particle vaccine primes immune responses preventing inactivated-virus vaccine-enhanced disease against respiratory syncytial virus. PMID: 28846899
Live-cell visualization of transmembrane protein oligomerization and membrane fusion using two-fragment haptoEGFP methodology. PMID: 22385277
Respiratory syncytial virus F and G proteins induce interleukin 1alpha, CC, and CXC chemokine responses by normal human bronchoepithelial cells. PMID: 20205592
Nonrecombining genes in a recombination environment: the Drosophila "dot" chromosome. PMID: 20940345
Improved epitope resolution of the prefusion trimer-specific antibody AM14 bound to the RSV F glycoprotein. PMID: 34420474
Structural interrogation of a trimeric prefusion RSV fusion protein vaccine candidate by a camelid nanobody. PMID: 37085457
Cross-Reactive Antigen Expressed by B6 Splenocytes Drives Receptor Editing and Marginal Zone Differentiation of IgG2a-Reactive AM14 Vkappa8 B Cells. PMID: 31534009
Genetic and cellular dissection of the activation of AM14 rheumatoid factor B cells in a mouse model of lupus. PMID: 25957308
Spontaneous loss of tolerance of autoreactive B cells in Act1-deficient rheumatoid factor transgenic mice. PMID: 23904159
The role of Bruton's tyrosine kinase in the development and BCR/TLR-dependent activation of AM14 rheumatoid factor B cells. PMID: 23804807
Glucose Requirement of Antigen-Specific Autoreactive B Cells and CD4+ T Cells. PMID: 36602759
A new site-directed transgenic rheumatoid factor mouse model demonstrates extrafollicular class switch and plasmablast formation. PMID: 20370572
Sympatry influence in the interaction of Trypanosoma cruzi with triatomine. PMID: 29160509
FcγRIIB regulation of BCR/TLR-dependent autoreactive B-cell responses. PMID: 20809520
A disease-related rheumatoid factor autoantibody is not tolerized in a normal mouse: implications for the origins of autoantibodies in autoimmune disease. PMID: 8879198
T cell-independent and toll-like receptor-dependent antigen-driven activation of autoreactive B cells. PMID: 18691914
RAGE-independent autoreactive B cell activation in response to chromatin and HMGB1/DNA immune complexes. PMID: 20014975
A multi-center study to determine genetic variations in the fusion gene of respiratory syncytial virus (RSV) from children <2 years of age in the U.S. PMID: 35792350
Diagnosis of Pancreatic Cancer Using miRNA30e Biosensor. PMID: 35781212
Characterization of a Prefusion-Specific Antibody That Recognizes a Quaternary, Cleavage-Dependent Epitope on the RSV Fusion Glycoprotein. PMID: 26161532
The relationship between gut microbiota and inflammatory response, learning and memory in mice by sleep deprivation. PMID: 37293204
The Respiratory Syncytial Virus (RSV) G Protein Enhances the Immune Responses to the RSV F Protein in an Enveloped Virus-Like Particle Vaccine Candidate. PMID: 36602367
Therapeutic effects of benznidazole in Swiss mice that are orally inoculated with Trypanosoma cruzi IV strains from the Western Brazilian Amazon. PMID: 34280400
Activation of rheumatoid factor-specific B cells is antigen dependent and occurs preferentially outside of germinal centers in the lupus-prone NZM2410 mouse model. PMID: 25015835
High affinity rheumatoid factor transgenic B cells are eliminated in normal mice. PMID: 9233605
Rheumatoid factor B cell memory leads to rapid, switched antibody-forming cell responses. PMID: 23365079
Activation of rheumatoid factor (RF) B cells and somatic hypermutation outside of germinal centers in autoimmune-prone MRL/lpr mice. PMID: 12727622
Differential cytokine production and bystander activation of autoreactive B cells in response to CpG-A and CpG-B oligonucleotides. PMID: 19864612
Genetic diversity for mycorrhizal symbiosis and phosphate transporters in rice. PMID: 26466747
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