一、中文名称：Anti-Flag Tag (DYKDDDDK) Antibody (M2)抗体
二、产品描述
FLAG标签，也被称为FLAG八肽或FLAG表位，是专为融合蛋白设计的首个表位标签，并且是唯一一个被授予专利的此类标签。这种标签的一个显著优势在于它可以应用于多种需要抗体识别的实验中。当没有特定蛋白质的专用抗体时，可以通过在该蛋白质上添加FLAG标签来利用抗FLAG序列的抗体对其进行研究。FLAG标签具有相对较小的分子量，仅为1012道尔顿（Da）。由于其结构中的多聚阴离子氨基酸成分，它对目标蛋白的功能影响极小。这使得FLAG标签成为一种理想的工具，因为它可以在不影响蛋白质原有活性的情况下进行标记和后续的研究工作。此外，FLAG标签能够支持高度特异性的pull-down实验，这类实验通常具有较低的非特异性背景信号，从而提高了检测结果的准确性和可靠性。
三、产品详情
名称：Anti-Flag Tag (DYKDDDDK) Antibody (M2) (RGK08501)
别名：Flag tag, Flag tag (DYKDDDDK-tag), Flag-tag, Flag peptide (DYKDDDDK)
货号：RGK08501
宿主：Mouse
同种型：IgG1, kappa
克隆类型：Monoclonal
靶标：Flag tag, Flag tag (DYKDDDDK-tag), Flag-tag, Flag peptide (DYKDDDDK)
浓度：1.56 mg/ml
内毒素水平：Please contact with the lab for this information.
纯度：>95% as determined by SDS-PAGE.
纯化方式：Protein A/G purified from cell culture supernatant.
克隆号：M2
应用：ChIP, ELISA, EIA, FCM, ICC, IF, IP, WB
状态：Liquid
保存溶液：0.01M PBS, pH 7.4.
稳定性和存储：Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. (RGK08501)
四、参考文献：
Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition. PMID: 33951064
An internal amino-terminal FLAG-tag octapeptide alters oligomerization of expressed surfactant protein-A. PMID: 32835791
Core-Shell Magnetic Imprinted Polymers for the Recognition of FLAG-Tagpeptide. PMID: 36834864
Proteomics-Based Analysis of Protein Complexes in Pluripotent Stem Cells and Cancer Biology. PMID: 27011181
An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments. PMID: 7530459
Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5. PMID: 9238646
A high-affinity monoclonal antibody against the FLAG tag useful for G-protein-coupled receptor study. PMID: 22465329
Tag-Specific Affinity Purification of Recombinant Proteins by Using Molecularly Imprinted Polymers. PMID: 30786715
Tandem Affinity Purification (TAP) of Interacting Prey Proteins with FLAG- and HA-Tagged Bait Proteins. PMID: 37450137
Hierarchically Imprinted Polymer for Peptide Tag Recognition Based on an Oriented Surface Epitope Approach. PMID: 32990425
High-level expression of anti FLAG tag antibody in plants. PMID: 38258646
Design and characterization of stimuli-responsive FLAG-tag analogues and the illumination-induced modulation of their interaction with antibody 4E11. PMID: 16889395
Using Affinity Pulldown Assays to Study Protein-Protein Interactions of Human NEIL1 Glycosylase and the Checkpoint Protein RAD9-RAD1-HUS1 (9-1-1) Complex. PMID: 37574484
Identification of Selenoprotein H Isoforms and Impact of Selenoprotein H Overexpression on Protein But Not mRNA Levels of 2 Other Selenoproteins in 293T Cells. PMID: 34510207
A novel in-capillary assay for dynamically monitoring fast binding between antibody and peptides using CE. PMID: 30367708
Synthesis, purification and bioactivity of recombinant human activin A expressed in the yeast Pichia pastoris. PMID: 19027859
Minimal FLAG sequence useful in the functional epitope tagging of H-Ras. PMID: 12074157
Lithobates catesbeianus (American Bullfrog) oocytes: a novel heterologous expression system for aquaporins. PMID: 29530931
Use of azobenzene amino acids as photo-responsive conformational switches to regulate antibody-antigen interaction. PMID: 19472287
Identification of amino acid residues responsible for the binding to anti-FLAG M2 antibody using a phage display combinatorial peptide library. PMID: 23807489
The human D1A dopamine receptor: heterologous expression in Saccharomyces cerevisiae and purification of the functional receptor. PMID: 9631523
Tetramerization domain of human butyrylcholinesterase is at the C-terminus. PMID: 9581552
Development of an efficiently cleaved, bioactive, highly pure FLAG-tagged recombinant human Mullerian Inhibiting Substance. PMID: 19755162
Production and purification of human indoleamine 2,3-dioxygenase (HuIDO) protein in a baculovirus expression system and production and characterization of egg yolk antibody against the purified HuIDO. PMID: 16174105
Characterization of capsid-modified adenovirus vectors containing heterologous peptides in the fiber knob, protein IX, or hexon. PMID: 17006548
Topology of the Na(+)/dicarboxylate cotransporter: the N-terminus and hydrophilic loop 4 are located intracellularly. PMID: 11248207
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