世界卫生组织于2024年8月12日发布的报告指出，全球猴痘疫情仍在持续。截至2024年6月30日，116个国家/领土/地区共向WHO报告了99176例实验室确诊病例和208例死亡。猴痘疫情再次构成“国际关注的突发公共卫生事件”。
值得注意的是，Clade Ib型猴痘病毒主要通过性接触传播，主要感染成年人；而近期发现Clade Ia型猴痘病毒则主要影响儿童，并通过多种传播方式传播。
6月新增26个国家报告了934例新增病例和4例死亡。东非的四个新国家（布隆迪、肯尼亚、卢旺达和乌干达）首次报告了猴痘病例，这些国家的病例均为Ⅰ型猴痘病毒。此外，科特迪瓦正在经历与II型猴痘病毒相关的疫情，南非报告了另外两例确诊病例。
Fig 1.2022年1月1日至2024年6月30日世卫组织从官方公开来源报告或确认的mpox确诊病例的地理分布
猴痘：从非洲森林到全球舞台
猴痘病毒（Mpox virus，MPXV），与天花病毒一样同属于痘病毒科（Poxviridae）的正痘病毒属（Orthopoxvirus）。这种病毒的形态特征鲜明，呈现圆角砖形或卵圆形，大小在200至400纳米之间。猴痘病毒粒子由脂蛋白包膜包裹，内部包含两个蛋白质侧体，以及一个含有大量双链DNA的厚膜核心。
Fig 2. 猴痘病毒电镜和结构图
猴痘病毒的核心蛋白包括A29L、A35R、B6R、M1R、H3L等，这些蛋白质在病毒的复制、结构稳定性和与宿主细胞的相互作用中发挥关键作用。病毒的基因组为双链DNA结构，长度约为197kb，基因组末端包含一个相同但方向相反的末端反向重复序列。这一复杂的基因组编码了约190个开放阅读框（ORFs），其中4个位于独特的末端反向重复序列（Terminal Inverted Repeats，TIRs）中。这些重复序列在病毒的基因组稳定性、复制和转录调控中扮演重要角色。
Fig 3. 猴痘病毒基因组图示
自2022年以来，猴痘疫情在多个国家爆发，不仅局限于传统流行区域，而且在欧洲、美洲、亚洲和中东等地出现病例，这标志着猴痘传播模式的显著变化。截至2024年6月，全球已有116个国家/领土/地区报告了猴痘病例，其中非洲区域报告的病例数最多，其次是美洲和欧洲区域。值得注意的是，刚果民主共和国报告了最多的猴痘病例，而其他国家如美国、巴西、西班牙、法国、哥伦比亚、墨西哥、英国、秘鲁、德国和澳大利亚也有较高的病例数。
Fig 4. 2022年1月1日至2024年6月30日世卫组织地区向世卫组织报告的每月实验室确认的mpox病例汇总流行曲线
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作为法国AntibodySystem亚洲总代理商，佰乐博生物提供猴痘病毒（Mpox)相关的蛋白/抗体/试剂盒产品，AntibodySystem团队对猴痘病毒(Mpox virus，Mpox)和痘苗病毒(vaccinia virus,VACV)的基因序列进行深度探索并成功设计开发真核系统表达的猴痘病毒(Mpox virus，Mpox)系列核心蛋白A29L、A35R、B6R、M1R、E8L以及相关具有生物学活性的中和抗体产品，助力疫苗、药物、诊断试剂等不同方向的研究。
真核表达猴痘核心蛋白及相关中和抗体
Monkeypox virus/MPXV A29L
A29L是细胞内成熟病毒（IMV）表面包膜蛋白（与牛痘病毒VACV,A27L同源）。溶瘤痘苗病毒(VV)属于痘病毒科中一拥有192 kb双螺旋DNA遗传物质的病毒,会在细胞质中繁殖出具有鞘膜的病毒,进而感染其他不同种的哺乳动物细胞及个体.VV中的A27L基因能表达出一14 kDa大小的鞘膜蛋白,此蛋白能与细胞表面上的硫酸乙酰肝素（Heparan Sulfate）作用使病毒跟细胞结合并且可催化细胞融合。
·Monkeypox virus/MPXV A29L
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Catalog No：EVV13401 |
Name:Recombinant Monkeypox virus/MPXV A29L Protein, C-His |
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Predicted molecular weight：15.33 kDa |
Expression system: Mammalian |
|
Protein length: Asp2-Glu110 |
Protein length: Asp2-Glu110 |
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SDS PAGE Purity：90% SDS PAGE for Recombinant Monkeypox virus/MPXV A29L Protein |
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·Anti-Monkeypox virus/MPXV A29L Antibody
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Catalog No：RVV13401 |
Name：Anti-Monkeypox virus/MPXV A29L Antibody (SAA0286） |
|
Host species：Mouse |
Applications：ELISA ,Neutralization |
|
Species reactivity: MPXV |
Stock：In stock |
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Detects Monkeypox virus/MPXV A29L indirect ELISAs |
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Monkeypox virus/MPXV A35R
A35R是细胞内包膜病毒粒子（IEV）和细胞外包膜病毒粒子（EEV）的包膜成分，与脊索痘病毒A33R同源。痘苗病毒中的A33R在病毒粒子在细胞间的传播中起着至关重要的作用，是疫苗开发的重要靶点，也有研究表明A35R是猴痘病毒(Monkeypox virus，MPXV)血清学检测的潜在靶点。
·Recombinant Monkeypox virus/MPXV A35R Protein
|
Catalog No：EVV13101 |
Name:Recombinant Monkeypox virus/MPXV A35R Protein, C-His |
|
Predicted molecular weight：16.65 kDa |
Expression system: Mammalian |
|
Protein length: Val57-Thr181 |
Stock：In stock |
|
SDS PAGE Purity：90% SDS PAGE for Recombinant Monkeypox virus/MPXV A35R Protein |
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·Anti-Monkeypox virus/MPXV A35R Antibody (SAA0287)
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Catalog No：RVV13101 |
Name:Anti-Monkeypox virus/MPXV A35R Antibody (SAA0287) |
|
Host species：Mouse |
Applications：ELISA ,Neutralization |
|
Species reactivity: MPXV |
Stock：In stock |
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Detects Monkeypox virus/MPXV A35R indirect ELISAs. |
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Monkeypox virus/MPXV B6R
B6R是宿主范围蛋白，位于细胞外包膜病毒粒子（EEV）的膜上，与牛痘病毒补体控制蛋白B5同源，参与补体激活的负调节。研究表明B5依赖于与A33或A34的相互作用，以定位到细胞内包膜的位点，并能相互结合释放到细胞外病毒体的包膜中。B5对于有效生产感染性EV至关重要，A33，A34，B5三者复合物在ER退出以及三种糖蛋白正确定位到包裹的细胞内部位中起作用。
·Recombinant Monkeypox virus/MPXV B6R/SL-159 Protein
|
Catalog No：EVV13501 |
Name:Recombinant Monkeypox virus/MPXV B6R/SL-159 Protein, C-His |
|
Predicted molecular weight：31.73 kDa |
Expression system: Mammalian |
|
Protein length: Thr20-His279 |
Stock：In stock |
|
SDS PAGE Purity：90% SDS PAGE for Recombinant Monkeypox virus/MPXV B6R/SL-159 Protein |
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Monkeypox virus/MPXV M1R
M1R与牛痘病毒L1蛋白同源，是一种在成熟IMV颗粒表面发现的跨膜蛋白。由L1R ORF编码，高度保守，参与病毒粒子组装，在病毒进入和成熟过程中发挥重要作用。L1是诱导低pH触发的细胞-细胞融合所必需的，且对牛痘病毒复制至关重要。
·Recombinant Monkeypox virus/MPXV M1R Protein
|
Catalog No：EVV13301 |
Name:Recombinant Monkeypox virus/MPXV M1R Protein, C-His |
|
Predicted molecular weight：22.35 kDa |
Expression system: Mammalian |
|
Protein length: Gly2-Gly183 |
Stock：In stock |
|
SDS PAGE Purity：90% SDS PAGE for Recombinant Monkeypox virus/MPXV M1R Protein |
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·Anti-Monkeypox virus/MPXV M1R Antibody
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Catalog No：RVV13301 |
Name:Anti-Monkeypox virus/MPXV M1R Antibody (SAA0283) |
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Host species：Mouse |
Applications：ELISA , Neutralization |
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Species reactivity: MPXV |
Stock：In stock |
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Detects Monkeypox virus/MPXV M1R indirect ELISAs.
|
|
Monkeypox virus/MPXV E8L
E8L是病毒入侵宿主细胞重要基因，在病毒入侵宿主完成复制的过程中作为MV的细胞表面结合蛋白与细胞表面的硫酸软骨素结合，以提供病毒粒子附着在靶细胞上。有研究表明利用siRNA技术能有效拮抗Monkeypox virus的A6R和E8L基因，由此构建siA6-a和si8 -d能破坏MPV复制。
·Recombinant Monkeypox virus/MPXV E8L Protein
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Catalog No：EVV13201 |
Name:Recombinant Monkeypox virus/MPXV E8L Protein, C-His |
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Predicted molecular weight：22.35 kDa |
Expression system: Mammalian |
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Protein length: Gly2-Gly183 |
Stock：In stock |
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SDS PAGE Purity：90% SDS PAGE for Recombinant Monkeypox virus/MPXV E8L Protein |
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·Anti-Monkeypox virus/MPXV E8L Antibody
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Catalog No：RVV13201 |
Name:Anti-Monkeypox virus/MPXV E8L Antibody (SAA0285) |
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Host species：Mouse |
Applications：ELISA , Neutralization |
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Species reactivity: MPXV |
Stock：In stock |
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Detects Monkeypox virus/MPXV E8L indirect ELISAs. |
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Monkeypox virus/MPXV H3L
H3L是一种324个AA长的糖基转移酶，位于病毒包膜上，在与宿主细胞的附着和随后的痘病毒进入中起关键作用。研究表明，天花疫苗诱导的抗体与H3蛋白结合，分化簇 (CD8)+ T细胞将H3L识别为抗原，表明其免疫学意义。
·Recombinant Monkeypox virus/MPXV H3L Protein
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Catalog No：EVV12701 |
Name:Recombinant Monkeypox virus/MPXV H3L Protein, C-His |
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Predicted molecular weight：35.46 kDa |
Expression system: Mammalian |
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Protein length: Ala2-Phe282 |
Stock：In stock |
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SDS PAGE Purity：90% SDS PAGE for Recombinant Monkeypox virus/MPXV H3L Protein |
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Catalog No. |
Product Name |
|
YVV12702 |
Recombinant MPXV H3L Protein, N-His |
|
EVV12703 |
Recombinant Monkeypox virus (MPXV) H3L Protein, C-Fc |
|
EVV12704 |
Recombinant MPXV H3L Protein, C-His |
|
YVV17101 |
Recombinant MPOX A5L Protein, N-GST & C-His |
|
YVV21901 |
Recombinant Monkeypox virus/MPXV A42R/Profilin Protein, N-His |
|
YVV17401 |
Recombinant Monkeypox virus/MPXV B16R Protein, N-His |
|
YVV17301 |
Recombinant Monkeypox virus/MPXV D6L Protein, N-His |
|
EVV17201 |
Recombinant Monkeypox virus/MPXV C15L Protein, C-His |
|
EVV17101 |
Recombinant Monkeypox virus/MPXV A5L Protein, C-His |
|
YVV16401 |
Recombinant Monkeypox virus/MPXV I1L Protein, N-His |
|
YVV16301 |
Recombinant Monkeypox virus/MPXV B21R Protein, N-His |
|
YVV13102 |
Recombinant Monkeypox virus/MPXV A35R Protein, N-GST & C-His |
|
YVV13402 |
Recombinant Monkeypox virus/MPXV A29L Protein, N-GST & C-His |
|
YVV15001 |
Recombinant Monkeypox virus/MPXV A30L Protein, N-His |
|
YVV14901 |
Recombinant Monkeypox virus/MPXV L1R Protein, N-His |
|
EVV12501 |
Recombinant Monkeypox virus/MPXV HA/B2R Protein, C-His |
|
YVV14801 |
Recombinant Monkeypox virus/MPXV F3L Protein, N-His |
|
YVV13601 |
Recombinant Monkeypox virus/MPXV D14L Protein, N-His |
|
YVV13701 |
Recombinant Monkeypox virus/MPXV A14L Protein, N-GST & C-His |
|
YVV13501 |
Recombinant Monkeypox virus/MPXV B6R/SL-159 Protein, N-His |
|
YVV13401 |
Recombinant Monkeypox virus/MPXV A29L Protein, N-His |
|
YVV13301 |
Recombinant Monkeypox virus/MPXV M1R Protein, N-His |
|
YVV13201 |
Recombinant Monkeypox virus/MPXV E8L Protein, N-His |
|
YVV13101 |
Recombinant Monkeypox virus/MPXV A35R Protein, N-His |
|
EVV13601 |
Recombinant Monkeypox virus/MPXV D14L Protein, C-His |
|
EVV13501 |
Recombinant Monkeypox virus/MPXV B6R/SL-159 Protein, C-His |
|
EVV13401 |
Recombinant Monkeypox virus/MPXV A29L Protein, C-His |
|
EVV13301 |
Recombinant Monkeypox virus/MPXV M1R Protein, C-His |
|
EVV13201 |
Recombinant Monkeypox virus/MPXV E8L Protein, C-His |
|
EVV13101 |
Recombinant Monkeypox virus/MPXV A35R Protein, C-His |
|
YVV13001 |
Recombinant Monkeypox virus/MPXV A27L Protein, N-His |
|
YVV12901 |
Recombinant Monkeypox virus/MPXV A26L Protein, N-GST & C-His |
|
YVV12801 |
Recombinant Monkeypox virus/MPXV C18L Protein, N-His |
|
YVV12701 |
Recombinant Monkeypox virus/MPXV H3L Protein, N-His |
|
YVV12601 |
Recombinant Monkeypox virus/MPXV A44R Protein, N-GST & C-His |
|
YVV12501 |
Recombinant Monkeypox virus/MPXV B2R/Hemagglutinin Protein, N-His |
|
YVV12401 |
Recombinant Monkeypox virus/MPXV D13L Protein, N-His |
|
YVV12301 |
Recombinant Monkeypox virus/MPXV C19L Protein, N-His |
|
YVV12201 |
Recombinant Monkeypox virus/MPXV A36R Protein, N-His |
|
YVV12101 |
Recombinant Monkeypox virus/MPXV B5R Protein, N-His |
|
YVV12001 |
Recombinant Monkeypox virus/MPXV A33R Protein, N-His |
|
EVV13302 |
Recombinant MPXV M1R Protein, C-His |
|
Catalog No. |
Product Name |
|
RVV13502 |
Anti-MPXV B6R/SL-159 Antibody (SAA2204) |
|
RVV13501 |
Anti-MPXV B6R/SL-159 Antibody (SAA2203) |
|
MVV12703 |
Anti-MPXV H3L Monoclonal Antibody (1A436) |
|
MVV12702 |
Anti-MPXV H3L Monoclonal Antibody (1A435) |
|
MVV12701 |
Anti-MPXV H3L Monoclonal Antibody (1A434) |
|
RVV13102 |
Anti-Monkeypox virus/MPXV A35R Antibody (SAA0287) |
|
RVV13418 |
Anti-Monkeypox virus/MPXV A29L Antibody (SAA0286) |
|
RVV13202 |
Anti-Monkeypox virus/MPXV E8L Antibody (SAA0285) |
|
RVV13304 |
Anti-Monkeypox virus/MPXV M1R Antibody (SAA0284) |
|
RVV13303 |
Anti-Monkeypox virus/MPXV M1R Antibody (SAA0283) |
|
RVV12702 |
Anti-Monkeypox virus/MPXV H3L Antibody (SAA0282) |
|
PVV21901 |
Anti-MPXV A42R/Profilin Polyclonal Antibody |
|
MVV13403 |
Anti-MPXV A29L Antibody (1A005) |
|
MVV13402 |
Anti-MPXV A29L Antibody (1A004) |
|
MVV13401 |
Anti-MPXV A29L Antibody (1A003) |
|
PVV16301 |
Anti-Monkeypox virus B21R Polyclonal Antibody |
|
PVV17101 |
Anti-MPOX A5L Polyclonal Antibody |
|
RVV13417 |
Anti-MPXV A29L Antibody (P2E12) |
|
RVV13416 |
Anti-MPXV A29L Antibody (P2H9) |
|
RVV13415 |
Anti-MPXV A29L Antibody (P2G9) |
|
RVV13414 |
Anti-MPXV A29L Antibody (P2B8) |
|
RVV13413 |
Anti-MPXV A29L Antibody (P2A6) |
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RVV13412 |
Anti-MPXV A29L Antibody (P2E5) |
|
RVV13411 |
Anti-MPXV A29L Antibody (P2H3) |
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RVV13410 |
Anti-MPXV A29L Antibody (P2G3) |
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RVV13409 |
Anti-MPXV A29L Antibody (P1H10) |
|
RVV13408 |
Anti-MPXV A29L Antibody (P1D7) |
|
RVV13407 |
Anti-MPXV A29L Antibody (P1B7) |
|
RVV13406 |
Anti-MPXV A29L Antibody (P1D4) |
|
RVV13405 |
Anti-MPXV A29L Antibody (P1F2) |
|
RVV13404 |
Anti-MPXV A29L Antibody (P1E2) |
|
RVV13403 |
Anti-MPXV A29L Antibody (P1A2) |
|
RVV13402 |
Anti-MPXV A29L Antibody (P1H1) |
|
RVV13501 |
Anti-MPXV B6R/SL-159 Antibody (SAA2203) |
|
RVV13502 |
Anti-MPXV B6R/SL-159 Antibody (SAA2204) |
|
PVV17401 |
Anti-Monkeypox virus/MPXV B16R Polyclonal Antibody |
|
PVV17301 |
Anti-Monkeypox virus/MPXV D6L Polyclonal Antibody |
|
PVV16401 |
Anti-Monkeypox virus/MPXV I1L Polyclonal Antibody |
|
PVV13401 |
Anti-Monkeypox virus/MPXV A29L Polyclonal Antibody |
|
PVV13101 |
Anti-Monkeypox virus/MPXV A35R Polyclonal Antibody |
|
PVV15001 |
Anti-Monkeypox virus/MPXV A30L Polyclonal Antibody |
|
PVV14901 |
Anti-Monkeypox virus/MPXV L1R Polyclonal Antibody |
|
PVV14801 |
Anti-Monkeypox virus/MPXV F3L Polyclonal Antibody |
|
PVV13601 |
Anti-Monkeypox virus/MPXV D14L Polyclonal Antibody |
|
PVV13701 |
Anti-Monkeypox virus/MPXV A14L Polyclonal Antibody |
|
PVV13501 |
Anti-Monkeypox virus/MPXV B6R/SL-159 Polyclonal Antibody |
|
PVV13301 |
Anti-Monkeypox virus/MPXV M1R Polyclonal Antibody |
|
PVV13201 |
Anti-Monkeypox virus/MPXV E8L Polyclonal Antibody |
|
PVV13001 |
Anti-Monkeypox virus/MPXV A27L Polyclonal Antibody |
|
PVV12901 |
Anti-Monkeypox virus/MPXV A26L Polyclonal Antibody |
|
PVV12801 |
Anti-Monkeypox virus/MPXV C18L Polyclonal Antibody |
|
PVV12701 |
Anti-Monkeypox virus/MPXV H3L Polyclonal Antibody |
|
PVV12601 |
Anti-Monkeypox virus/MPXV A44R Polyclonal Antibody |
|
PVV12501 |
Anti-Monkeypox virus/MPXV B2R/HA/Hemagglutinin Polyclonal Antibody |
|
PVV12401 |
Anti-Monkeypox virus/MPXV D13L Polyclonal Antibody |
|
PVV12301 |
Anti-Monkeypox virus/MPXV C19L Polyclonal Antibody |
|
PVV12201 |
Anti-Monkeypox virus/MPXV A36R Polyclonal Antibody |
|
PVV12101 |
Anti-Monkeypox virus/MPXV B5R Polyclonal Antibody |
|
PVV12001 |
Anti-Monkeypox virus/MPXV A33R Polyclonal Antibody |
|
RVV13101 |
Mouse Anti-Monkeypox virus/MPXV A35R Antibody |
|
RVV13401 |
Mouse Anti-Monkeypox virus/MPXV A29L Antibody |
|
RVV13201 |
Mouse Anti-Monkeypox virus/MPXV E8L Antibody |
|
RVV13302 |
Mouse Anti-Monkeypox virus/MPXV M1R Antibody |
|
RVV13301 |
Mouse Anti-Monkeypox virus/MPXV M1R Antibody |
|
RVV12701 |
Mouse Anti-Monkeypox virus/MPXV H3L Antibody |
|
Catalog No. |
Product Name |
|
KAV13402 |
Anti-MPXV A29L Human IgG ELISA Kit |
|
KAV13403 |
Anti-MPXV A29L Human IgM ELISA Kit |
|
KAV13404 |
Anti-MPXV A29L Human IgA ELISA Kit |
|
KAV13102 |
Anti-MPXV A35R Human IgG ELISA Kit |
|
KAV13103 |
Anti-MPXV A35R Human IgM ELISA Kit |
|
KAV13104 |
Anti-MPXV A35R Human IgA ELISA Kit |
|
KAV13302 |
Anti-MPXV M1R Human IgG ELISA Kit |
|
KAV13303 |
Anti-MPXV M1R Human IgM ELISA Kit |
|
KAV13304 |
Anti-MPXV M1R Human IgA ELISA Kit |
|
KAV13202 |
Anti-MPXV E8L Human IgG ELISA Kit |
|
KAV13203 |
Anti-MPXV E8L Human IgM ELISA Kit |
|
KAV13204 |
Anti-MPXV E8L Human IgA ELISA Kit |
|
KAV12702 |
Anti-MPXV H3L Human IgG ELISA Kit |
|
KVV13501 |
Monkeypox Virus (MPXV) B6R Antigen ELISA Kit |
|
KVV13401 |
Monkeypox Virus (MPXV) A29L Antigen ELISA Kit |
|
KVV13402 |
Monkeypox Virus (MPXV) A29L Antigen ELISA Kit-HS |
|
KVV13101 |
Monkeypox Virus (MPXV) A35R Antigen ELISA Kit |
|
KVV13301 |
Monkeypox Virus (MPXV) M1R Antigen ELISA Kit |
|
KVV13201 |
Monkeypox Virus (MPXV) E8L Antigen ELISA Kit |
|
KVV12701 |
Monkeypox Virus (MPXV) H3L Antigen ELISA Kit |
参考文献：
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